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Clinical and Diagnostic Laboratory Immunology, January 1999, p. 79-84, Vol. 6, No. 1
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Dysregulated Synthesis of Intracellular Type 1 and Type 2 Cytokines by T Cells of Patients with Cutaneous T-Cell Lymphoma

Bang-Ning Lee,1 Madeleine Duvic,2 Chih-Kwang Tang,1 Carlos Bueso-Ramos,1 Zeev Estrov,3 and James M. Reuben1,*

Division of Pathology and Laboratory Medicine1 and Section of Dermatology2 and Department of Bioimmunotherapy,3 Division of Medicine, The University of Texas M. D. Anderson Cancer Center, Houston, Texas

Received 30 April 1998/Returned for modification 24 June 1998/Accepted 7 October 1998

Mycosis fungoides (MF) and Sezary syndrome (SS) are the two main clinical entities of cutaneous T-cell lymphoma (CTCL). As the disease progresses from MF to SS, a switch from a type 1 (interleukin [IL]-2 and gamma interferon [IFN-gamma ]) to a type 2 (IL-4) cytokine production profile occurs. Although roles for type 1 and type 2 cytokines in the pathogenesis of CTCL have been proposed, the cellular origins of these cytokines are unclear. Using flow cytometry to identify individual T-cell subsets, we studied cytokine synthesis by the T cells of 13 patients with SS and 12 with MF and 9 hematologically healthy donors. Upon activation with phorbol 12-myristate 13-acetate (PMA), the numbers of T cells synthesizing IL-2 were similar for all study groups. Whereas the predominant T-cell producing IL-2 in healthy donors and in those with MF was CD7+, in patients with SS, it was CD7-. Although the number of IL-4+ CD4+ T cells was low for all study groups, there was a significantly higher number of IL-4+ CD8+ T cells in patients with MF than in those with SS or healthy donors. There was a decline in the number of IFN-gamma -producing T cells in CTCL donors compared to that in healthy donors. More importantly, there was a significant decrease in the number of IFN-gamma -producing T cells with disease progression from MF to SS. The inability of these T cells to synthesize IFN-gamma may have prognostic value in CTCL, since it may be responsible for the progression of the disease from MF to SS.

* Corresponding author. Mailing address: Division of Pathology and Laboratory Medicine, Box 7, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030. Phone: (713) 792-3559. Fax: (713) 792-4296. E-mail: jreuben{at}notes.mdacc.tmc.edu.

Clinical and Diagnostic Laboratory Immunology, January 1999, p. 79-84, Vol. 6, No. 1
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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