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Clinical and Diagnostic Laboratory Immunology, 09 1995, 531-534, Vol 2, No. 5
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Monoclonal antibodies to Coxiella burnetii that cross-react with strain Nine Mile

Z Sekeyova, E Kovacova, J Kazar, R Toman and S Olvecka
Department of Rickettsiae, Slovak Academy of Sciences, Bratislava.

Results are presented to show the binding properties of five monoclonal antibodies directed to Coxiella burnetii Priscilla with cross-reactions to the Nine Mile strain. The monoclonal antibodies preferentially recognize phase I epitopes by ELISA and recognize phase II epitopes by immunoblotting but do not allow differentiation between so-called chronic and acute strains of C. burnetii. The only difference in reactivity was in the staining pattern revealed after reactions with lipopolysaccharide I antigens.