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Clinical and Vaccine Immunology, September 2009, p. 1309-1313, Vol. 16, No. 9
1071-412X/09/$08.00+0     doi:10.1128/CVI.00028-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Single-Antigen Serological Testing for Bovine Tuberculosis{triangledown}

Lawrence R. Green,1 Cynthia C. Jones,1 Anne L. Sherwood,1 Inna V. Garkavi,1 Gerard A. Cangelosi,1 Tyler C. Thacker,2 Mitchell V. Palmer,2 W. Ray Waters,2 and Chris V. Rathe1*

PriTest Inc., Redmond, Washington,1 National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa2

Received 20 January 2009/ Returned for modification 27 February 2009/ Accepted 6 July 2009

Antibody responses are useful indicators of Mycobacterium bovis infection of cattle. Tests for such responses often use multiple M. bovis antigens as detection probes. This is recommended because responses to single antigens may be too variable for consistent diagnosis. However, the use of multiple antigens increases costs and the risk of false-positive results. As an alternative, the SeraLyte-Mbv system detects responses to a single M. bovis antigen, MPB83, by using a chemiluminescent testing platform with a high degree of analytical sensitivity. Testing with the SeraLyte-Mbv system was conducted in a blinded fashion with sera from experimentally infected and control cattle. To assess the species specificity of the single-antigen test, the sample included sera from animals infected with M. bovis (n = 27), M. kansasii (n = 4), M. avium subsp. paratuberculosis (n = 11), M. avium subsp. avium (n = 12), and uninfected animals (n = 15). Upon unblinding of the results, the sensitivity of the SeraLyte-Mbv system relative to the results for animals with known M. bovis infection was 89%. Consistent with the conservation of MPB83 sequences within the genus Mycobacterium, all 4 M. kansasii-infected animals tested positive with the SeraLyte-Mbv system and all 23 M. avium-infected animals tested negative. Blinded analysis of 30 serum samples collected from nine animals at various time points postinfection indicated 100% sensitivity after ≥3 months postinfection. All 15 uninfected samples in the blinded sample set tested negative with the SeraLyte-Mbv system. Unblinded analysis of sera from an additional 895 animals in 10 accredited bovine tuberculosis-free states revealed 98% specificity overall. The results support the feasibility of single-antigen testing for bovine tuberculosis with the SeraLyte-Mbv system.

* Corresponding author. Mailing address: PriTest Inc., 2897 152nd Avenue NE, Redmond, WA 98052. Phone: (425) 869-6500. Fax: (425) 869-4231. E-mail: crathe{at}pritest.com

{triangledown} Published ahead of print on 15 July 2009.

Clinical and Vaccine Immunology, September 2009, p. 1309-1313, Vol. 16, No. 9
1071-412X/09/$08.00+0     doi:10.1128/CVI.00028-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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