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Clinical and Vaccine Immunology, September 2009, p. 1279-1284, Vol. 16, No. 9
1071-412X/09/$08.00+0     doi:10.1128/CVI.00143-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Evaluation of the Specificity of Pneumococcal Polysaccharide Enzyme-Linked Immunosorbent Assay and the Effect of Serum Adsorption Based on Standard Pneumococcal Serogroup- or Serotype-Specific Rabbit Antisera{triangledown}

Hans-Christian Slotved,* Christina Guttmann, Charlotte Demuth Pedersen, Jasper Neergaard Jacobsen, and Karen Angeliki Krogfelt

Department of Bacteriology, Mycology and Parasitology, Statens Serum Institut, Copenhagen, Denmark

Received 31 March 2009/ Returned for modification 24 April 2009/ Accepted 29 June 2009

Worldwide, Streptococcus pneumoniae (pneumococcus) is a major cause of morbidity and mortality, especially in infants and elderly people. Pneumococcal capsular polysaccharides are well characterized, and more than 90 different serotypes have been identified. Serotype-specific antibodies against the capsular polysaccharide are produced during infection. Detection of antibodies against pneumococci by enzyme-linked immunosorbent assay (ELISA) is performed according to WHO guidelines, using antigens provided by ATCC. However, testing the ELISA for specificity is challenging due to the difficulty in obtaining human naïve serum with pneumococcal antibodies as well as human serum with antibodies against a single serotype. The application of well-defined serotype-specific sera produced in animals to evaluate the specificity of the ATCC antigens and the effect of adsorption with cell wall and 22F polysaccharides has not been performed before, to our knowledge. In this study, the specificity of ATCC antigens (serotypes 4, 6B, 9V, 14, 18C, 19F, and 23F) was tested by using commercial serotype-, serogroup-, and pool-specific pneumococcal rabbit antisera.

* Corresponding author. Mailing address: Department of Bacteriology, Mycology and Parasitology, Division of Microbiology and Diagnostics, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen, Denmark. Phone: 45 32688422. Fax: 45 32688238. E-mail: hcs{at}ssi.dk

{triangledown} Published ahead of print on 8 July 2009.

Clinical and Vaccine Immunology, September 2009, p. 1279-1284, Vol. 16, No. 9
1071-412X/09/$08.00+0     doi:10.1128/CVI.00143-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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