This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Sánchez-Aparicio, M. T.
Right arrow Articles by Sobrino, F.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sánchez-Aparicio, M. T.
Right arrow Articles by Sobrino, F.

 Previous Article  |  Next Article 

Clinical and Vaccine Immunology, August 2009, p. 1228-1235, Vol. 16, No. 8
1071-412X/09/$08.00+0     doi:10.1128/CVI.00139-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Discriminating Foot-and-Mouth Disease Virus-Infected and Vaccinated Animals by Use of β-Galactosidase Allosteric Biosensors{triangledown} ,{dagger}

M. Teresa Sánchez-Aparicio,1 María Flora Rosas,1 Rosa Maria Ferraz,2,3 Laura Delgui,1,{ddagger} Juan J. Veloso,4 Esther Blanco,5 Antonio Villaverde,2,3 and Francisco Sobrino1,5*

Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid,1 Institut de Biotecnologia i de Biomedicina, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona,2 CIBER en Bioingenería, Biomateriales y Nanomedicina, Bellaterra, Barcelona,3 Tecnología para Diagnóstico e Investigación, Alcobendas, 28100 Madrid,4 CISA-INIA, Valdeolmos, 28130 Madrid, Spain5

Received 30 March 2009/ Returned for modification 22 April 2009/ Accepted 10 June 2009

Recombinant β-galactosidases accommodating one or two different peptides from the foot-and-mouth disease virus (FMDV) nonstructural protein 3B per enzyme monomer showed a drastic enzymatic activity reduction, which mainly affected proteins with double insertions. Recombinant β-galactosidases were enzymatically reactivated by 3B-specific murine monoclonal and rabbit polyclonal antibodies. Interestingly, these recombinant β-galactosidases, particularly those including one copy of each of the two 3B sequences, were efficiently reactivated by sera from infected pigs. We found reaction conditions that allowed differentiation between sera of FMDV-infected pigs, cattle, and sheep and those of naïve and conventionally vaccinated animals. These FMDV infection-specific biosensors can provide an effective and versatile alternative for the serological distinction of FMDV-infected animals.

* Corresponding author. Mailing address: CBMSO, UAM, 28040 Madrid, Spain. Phone: 34 - 91 - 1964494. Fax: 34 - 91 - 1964420. E-mail: fsobrino{at}cbm.uam.es

{triangledown} Published ahead of print on 24 June 2009.

{dagger} Supplemental material for this article may be found at http://cvi.asm.org/.

{ddagger} Present address: Department of Molecular and Cellular Biology, Centro Nacional de Biotecnología-CSIC, Cantoblanco, 28049 Madrid, Spain.

Clinical and Vaccine Immunology, August 2009, p. 1228-1235, Vol. 16, No. 8
1071-412X/09/$08.00+0     doi:10.1128/CVI.00139-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»