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Clinical and Vaccine Immunology, August 2009, p. 1132-1138, Vol. 16, No. 8
1071-412X/09/$08.00+0     doi:10.1128/CVI.00163-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Characterization of Monoclonal Antibodies to Junin Virus Nucleocapsid Protein and Application to the Diagnosis of Hemorrhagic Fever Caused by South American Arenaviruses{triangledown}

Mina Nakauchi,1 Shuetsu Fukushi,1 Masayuki Saijo,1 Tetsuya Mizutani,1 Agustín E. Ure,2 Victor Romanowski,2 Ichiro Kurane,1 and Shigeru Morikawa1*

Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan,1 Facultad de Ciencias Exactas, Instituto de Biotecnología y Biología Molecular, Universidad Nacional de La Plata, Consejo Nacional de Investigaciones Científicas y Técnicas (IBBM-UNLP-CONICET-CCT La Plata), La Plata, Argentina2

Received 13 April 2009/ Returned for modification 26 May 2009/ Accepted 10 June 2009

Junin virus (JUNV), Machupo virus, Guanarito virus, Sabia virus, and Chapare virus are members of New World arenavirus clade B and are the etiological agents of viral hemorrhagic fevers that occur in South America. In this study, we produced three monoclonal antibodies (MAbs) to the recombinant nucleocapsid protein of JUNV, designated C6-9, C11-12, and E4-2. The specificity of these MAbs was examined by enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence assay, and an epitope-mapping method. Using these MAbs, we developed antigen (Ag) capture ELISA systems. We showed that by using MAb C6-9, JUNV Ag was specifically detected. On the other hand, by using MAb C11-12 or E-4-2, the Ags of all human pathogenic South American arenaviruses were detected. The combined use of these Ag capture ELISA systems in the present study may be useful for the diagnosis of acute-phase viral hemorrhagic fever due to infection by a South American arenavirus.

* Corresponding author. Mailing address: Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan. Phone: 44-42-561-0771. Fax: 44-42-561-2039. E-mail: morikawa{at}nih.go.jp

{triangledown} Published ahead of print on 24 June 2009.

Clinical and Vaccine Immunology, August 2009, p. 1132-1138, Vol. 16, No. 8
1071-412X/09/$08.00+0     doi:10.1128/CVI.00163-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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