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Clinical and Vaccine Immunology, June 2009, p. 944-948, Vol. 16, No. 6
1071-412X/09/$08.00+0     doi:10.1128/CVI.00087-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Identification of Secreted Antigen 3 from Babesia gibsoni{triangledown}

Honglin Jia,1 M. Alaa Terkawi,1 Gabriel Oluga Aboge,1 Youn-Kyoung Goo,1 Liqing Ma,1,2 Jinlin Zhou,1,3 Yoshifumi Nishikawa,1 Ikuo Igarashi,1 Kozo Fujisaki,1 and Xuenan Xuan1*

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan,1 Qinghai Academy of Animal and Veterinary Science, Weier Road, Xining, Qinghai 810003, China,2 Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Ziyue Road, Minhang District, Shanghai 200241, China3

Received 1 March 2009/ Returned for modification 20 March 2009/ Accepted 15 April 2009

A cDNA expression library of Babesia gibsoni was screened with the serum collected from a dog experimentally infected with B. gibsoni. A novel antigen sharing homology with secreted antigen 1 of B. gibsoni was isolated. The genomic analysis indicated that the BgSA3 gene exists as multicopies in the genome of B. gibsoni. The putative peptide encoded by the BgSA3 gene showed some characteristics of secreted proteins. The serum raised in mice immunized with the recombinant BgSA3 expressed in Escherichia coli could recognize a native parasite protein with a molecular mass of 70 kDa. Moreover, a sandwich enzyme-linked immunosorbent assay with anti-BgSA3 antibodies could detect the circulating BgSA3 in the blood plasma of dogs experimentally infected with B. gibsoni. The identification of BgSA3 provided a useful target for the development of a diagnostic test for detecting specific antibodies and circulating antigens.

* Corresponding author. Mailing address: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan. Phone: 81-155-49-5648. Fax: 81-155-49-5643. E-mail: gen{at}obihiro.ac.jp

{triangledown} Published ahead of print on 22 April 2009.

Clinical and Vaccine Immunology, June 2009, p. 944-948, Vol. 16, No. 6
1071-412X/09/$08.00+0     doi:10.1128/CVI.00087-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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