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Clinical and Vaccine Immunology, June 2009, p. 792-797, Vol. 16, No. 6
1071-412X/09/$08.00+0     doi:10.1128/CVI.00032-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Immunization with Oligomannose-Coated Liposome-Entrapped Dense Granule Protein 7 Protects Dams and Offspring from Neospora caninum Infection in Mice {triangledown}

Yoshifumi Nishikawa,1,{dagger} Houshuang Zhang,1,{dagger} Yuzuru Ikehara,2 Naoya Kojima,3 Xuenan Xuan,1 and Naoaki Yokoyama1*

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan,1 Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8568, Japan,2 Institute of Glycoscience, Tokai University, Hiratsuka, Kanagawa 259-1292, Japan3

Received 22 January 2009/ Returned for modification 2 March 2009/ Accepted 31 March 2009

The present study demonstrates that the subcutaneous administration of Neospora caninum dense granule protein 7 (NcGRA7) entrapped in liposomes coated with mannotriose strongly induces the parasite-specific T-helper type 1 immune response and humoral antibody in mice. Although anti-NcGRA7 immunoglobulin G1 antibody production was induced in mice injected with NcGRA7 alone, the dams and offspring were never protected from N. caninum infection. The immunization of mice with liposome-entrapped NcGRA7 before pregnancy resulted in increased offspring survival and decreased the infection rates in the brains of dams after parasite infection at 6 to 9 days of gestation. In conclusion, oligomannose-coated liposome-entrapped NcGRA7 can be used as a new type of effective vaccine to control neosporosis.

* Corresponding author. Mailing address: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan. Phone: 81-155-49-5649. Fax: 81-155-49-5643. E-mail: yokoyama{at}obihiro.ac.jp

{triangledown} Published ahead of print on 8 April 2009.

{dagger} Y.N. and H.Z. contributed equally to this work.

Clinical and Vaccine Immunology, June 2009, p. 792-797, Vol. 16, No. 6
1071-412X/09/$08.00+0     doi:10.1128/CVI.00032-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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