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Clinical and Vaccine Immunology, May 2009, p. 667-671, Vol. 16, No. 5
1071-412X/09/$08.00+0     doi:10.1128/CVI.00043-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Enzyme-Linked Immunosorbent Assay Using Glycoprotein and Monoclonal Antibody for Detecting Antibodies to Vesicular Stomatitis Virus Serotype New Jersey{triangledown}

Hyang-Sim Lee,1,2 Eun-Jeong Heo,1 Hye-Young Jeoung,1 Hyo-Rim Ko,1 Chang-Hee Kweon,1 Hee-Jeong Youn,2 and Young-Joon Ko1*

National Veterinary Research and Quarantine Service, Anyang, Gyeonggi-do 430-824,1 College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea2

Received 30 January 2009/ Returned for modification 23 February 2009/ Accepted 3 March 2009

In this study, an enzyme-linked immunosorbent assay (ELISA) using glycoprotein and a monoclonal antibody (MAb) was developed for the detection of antibodies to vesicular stomatitis virus (VSV) serotype New Jersey (NJ). The glycoprotein to be used as a diagnostic antigen was extracted from partially purified VSV-NJ, and a neutralizing MAb specific to VSV-NJ was incorporated to compete with antibodies in a blocking ELISA using glycoprotein (GP ELISA). The cutoff of the GP ELISA was set at 40% inhibition, which corresponded to a virus neutralization test (VNT) titer of 32. With this threshold, the GP ELISA exhibited 99.6% specificity for naïve sera (n = 3,005) from cattle (n = 1,040), pigs (n = 1,120), and horses (n = 845) from domestic farms. The GP ELISA did not cross-react with sera positive for foot-and-mouth disease virus, swine vesicular disease virus, or VSV serotype Indiana. The GP ELISA was more compatible with the VNT than was the nucleocapsid-based ELISA for VSV-NJ-positive sera (n = 19). Taken together, this GP ELISA could be a useful tool as an alternative to the VNT for detecting antibodies specific to VSV-NJ.

* Corresponding author. Mailing address: National Veterinary Research and Quarantine Service, Anyang-6-dong, Anyang, Gyeonggi-do 430-824, Republic of Korea. Phone: 82 31 467 1858. Fax: 82 31 449 5882. E-mail: koyj{at}nvrqs.go.kr

{triangledown} Published ahead of print on 11 March 2009.

Clinical and Vaccine Immunology, May 2009, p. 667-671, Vol. 16, No. 5
1071-412X/09/$08.00+0     doi:10.1128/CVI.00043-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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