This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Campbell, D. E.
Right arrow Articles by Douglas, S. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Campbell, D. E.
Right arrow Articles by Douglas, S. D.

 Previous Article

Clinical and Vaccine Immunology, April 2009, p. 594-596, Vol. 16, No. 4
1071-412X/09/$08.00+0     doi:10.1128/CVI.00406-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Novel Method for Determination of Substance P Levels in Unextracted Human Plasma by Using Acidification{triangledown}

Donald E. Campbell,* Paula Bruckner, Nancy B. Tustin, Richard Tustin III, and Steven D. Douglas

Division of Allergy and Immunology, Joseph Stokes Jr. Research Institute at the Children's Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania

Received 7 November 2008/ Returned for modification 3 December 2008/ Accepted 26 January 2009

Substance P (SP) is a member of the tachykinin family and has an important role in immune responses. SP is detectable in plasma in a free and bound state. Simple modification of a commercially available SP enzyme-linked immunosorbent assay allows the dissociation and capture of plasma SP without solid-phase extraction.

* Corresponding author. Mailing address: Division of Allergy and Immunology, The Children's Hospital of Philadelphia, 34th and Civic Center Blvd., Philadelphia, PA 19104. Phone: (215) 590-2353. Fax: (215) 590-3044. E-mail: campbelld{at}email.chop.edu

{triangledown} Published ahead of print on 4 February 2009.

Clinical and Vaccine Immunology, April 2009, p. 594-596, Vol. 16, No. 4
1071-412X/09/$08.00+0     doi:10.1128/CVI.00406-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»