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Clinical and Vaccine Immunology, April 2009, p. 551-557, Vol. 16, No. 4
1071-412X/09/$08.00+0     doi:10.1128/CVI.00459-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Peripheral Ovine Progressive Pneumonia Provirus Levels Correlate with and Predict Histological Tissue Lesion Severity in Naturally Infected Sheep{triangledown}

Lynn M. Herrmann-Hoesing,1,2* Susan M. Noh,1,2 Stephen N. White,1,2,3 Kevin R. Snekvik,2 Thomas Truscott,1 and Donald P. Knowles1,2

Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164-6630,1 Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040,2 Center for Integrated Biotechnology, Washington State University, Pullman, Washington 991643

Received 5 December 2008/ Returned for modification 6 February 2009/ Accepted 19 February 2009

Studies were undertaken to determine whether anti-ovine progressive pneumonia virus (OPPV) antibody responses in serum or OPP provirus levels in peripheral blood associate with the degree of histologically measured tissue lesions in naturally OPPV-infected sheep. Sections of formalin-fixed, paraffin-embedded, and hematoxylin- and eosin-stained lung, mammary gland, carpal synovial membrane, and brain tissues from 11 OPPV-infected ewes (mean age of 8.6 years) and 5 OPPV-uninfected ewes (mean age of 6 years) were evaluated for lesion severity. Ovine progressive pneumonia (OPP) provirus levels and anti-OPPV antibody titers in peripheral blood and serum samples, respectively, were measured upon euthanasia and 3 years prior to euthanasia. Both mean peripheral OPP provirus levels and mean serum anti-surface envelope glycoprotein (anti-SU) antibody titers at the time of euthanasia were significantly higher in ewes with moderate to severe histological lesions than in ewes with no to mild histological lesions. However, although mean peripheral blood OPP provirus levels at euthanasia and 3 years prior to euthanasia significantly correlated with the highest histological lesion score for any affected tissue (two-tailed P values, 0.03 and 0.02), mean serum anti-SU antibody titers, anti-capsid antibody titers, and anti-transmembrane 90 antibody titers at euthanasia did not show a significant correlation with the highest histological lesion score for any tissue (two-tailed P values, 0.32, 0.97, and 0.18, respectively). These data are the first to show that OPP provirus levels predict and correlate with the extent of OPPV-related histological lesions in various OPPV-affected tissues. These findings suggest that peripheral OPP provirus levels quantitatively contribute more to the development of histological lesions than the systemic anti-SU antibody host immune response.

* Corresponding author. Mailing address: Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, WA 99164-6630. Phone: (509) 335-6068. Fax: (509) 335-8328. E-mail: lherrman{at}vetmed.wsu.edu

{triangledown} Published ahead of print on 4 March 2009.

Clinical and Vaccine Immunology, April 2009, p. 551-557, Vol. 16, No. 4
1071-412X/09/$08.00+0     doi:10.1128/CVI.00459-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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