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Clinical and Vaccine Immunology, April 2009, p. 544-550, Vol. 16, No. 4
1071-412X/09/$08.00+0 doi:10.1128/CVI.00339-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Immunogenicity of a Whole-Cell Pertussis Vaccine with Low Lipopolysaccharide Content in Infants
Tatiane Queiroz Zorzeto,1,4
Hisako Gondo Higashi,2
Marcos Tadeu Nolasco da Silva,1,4
Emilia de Faria Carniel,1,4
Waldely Oliveira Dias,2
Vanessa Domingues Ramalho,1
Taís Nitsch Mazzola,1
Simone Corte Batista Souza Lima,1
André Moreno Morcillo,1,4
Marco Antonio Stephano,3
Maria Ângela Reis de Góes Antonio,4
Maria de Lurdes Zanolli,4
Isaias Raw,2 and
Maria Marluce dos Santos Vilela1,4*
Center for Investigation in Pediatrics,1 Pediatrics Department, State University of Campinas Medical School, Rua Tessália Vieira de Camargo 126, Campinas, São Paulo, Brazil CEP 13083-887,4 Butantan Institute, Rua Vital Brasil, 1500, São Paulo, São Paulo, Brazil CEP 05503-900,2 Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Prof. Lineu Prestes 580, Butantã, São Paulo, Brazil CEP 05508-0003
Received 17 September 2008/ Returned for modification 3 November 2008/ Accepted 10 February 2009
The lack of a clear correlation between the levels of antibody to pertussis antigens and protection against disease lends credence to the possibility that cell-mediated immunity provides primary protection against disease. This phase I comparative trial had the aim of comparing the in vitro cellular immune response and anti-pertussis toxin (anti-PT) immunoglobulin G (IgG) titers induced by a cellular pertussis vaccine with low lipopolysaccharide (LPS) content (wPlow vaccine) with those induced by the conventional whole-cell pertussis (wP) vaccine. A total of 234 infants were vaccinated at 2, 4, and 6 months with the conventional wP vaccine or the wPlow vaccine. Proliferation of CD3+ T cells was evaluated by flow cytometry after 6 days of peripheral blood mononuclear cell culture with stimulation with heat-killed Bordetella pertussis or phytohemagglutinin (PHA). CD3+, CD4+, CD8+, and T-cell receptor 
-positive (+) cells were identified in the gate of blast lymphocytes. Gamma interferon, tumor necrosis factor alpha, interleukin-4 (IL-4), and IL-10 levels in supernatants and serum anti-PT IgG levels were determined using enzyme-linked immunosorbent assay (ELISA). The net percentage of CD3+ blasts in cultures with B. pertussis in the group vaccinated with wP was higher than that in the group vaccinated with the wPlow vaccine (medians of 6.2% for the wP vaccine and 3.9% for the wPlow vaccine; P = 0.029). The frequencies of proliferating CD4+, CD8+, and + cells, cytokine concentrations in supernatants, and the geometric mean titers of anti-PT IgG were similar for the two vaccination groups. There was a significant difference between the T-cell subpopulations for B. pertussis and PHA cultures, with a higher percentage of + cells in the B. pertussis cultures (P < 0.001). The overall data did suggest that wP vaccination resulted in modestly better specific CD3+ cell proliferation, and + cell expansions were similar with the two vaccines.
* Corresponding author. Mailing address: Center for Investigation in Pediatrics and Pediatrics Department, State University of Campinas Medical School, Rua Tessália Vieira de Camargo 126, Campinas, São Paulo, Brazil CEP 13083-887. Phone: 55-19-35218963. Fax: 55-19-35218972. E-mail: marluce{at}fcm.unicamp.br
Published ahead of print on 4 March 2009.
Clinical and Vaccine Immunology, April 2009, p. 544-550, Vol. 16, No. 4
1071-412X/09/$08.00+0 doi:10.1128/CVI.00339-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.
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