This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Barefoot, B. E.
Right arrow Articles by Ramsburg, E. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barefoot, B. E.
Right arrow Articles by Ramsburg, E. A.

 Previous Article  |  Next Article 

Clinical and Vaccine Immunology, April 2009, p. 488-498, Vol. 16, No. 4
1071-412X/09/$08.00+0     doi:10.1128/CVI.00451-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Recombinant Vesicular Stomatitis Virus Expressing Influenza Nucleoprotein Induces CD8 T-Cell Responses That Enhance Antibody-Mediated Protection after Lethal Challenge with Influenza Virus{triangledown}

Brice E. Barefoot, Christopher J. Sample, and Elizabeth A. Ramsburg*

Duke University Human Vaccine Institute, Department of Medicine, Duke University School of Medicine, Durham, North Carolina 27710

Received 2 December 2008/ Returned for modification 26 January 2009/ Accepted 15 February 2009

Live attenuated vaccine vectors based on recombinant vesicular stomatitis viruses (rVSVs) expressing foreign antigens are highly effective vaccines in animal models. In this study, we report that an rVSV expressing influenza nucleoprotein (VSV NP) from the first position of the VSV genome induces robust anti-NP CD8 T cells in immunized mice. These CD8 T cells are phenotypically similar to those induced by natural influenza infection and are cytotoxic in vivo. Animals immunized with an rVSV expressing the influenza hemagglutinin (rVSV HA) were protected but still exhibited considerable morbidity after challenge. Animals receiving a cocktail vaccine of rVSV NP and rVSV HA had reduced pulmonary viral loads, less weight loss, and reduced clinical signs of illness after influenza virus challenge, relative to those vaccinated with rVSV HA alone. Influenza NP is a highly conserved antigen, and induction of protective anti-NP responses may be a productive strategy for generating heterologous protection against divergent influenza strains.

* Corresponding author. Mailing address: Duke University Medical Center, Global Health Research Building, Room 1044, 102 Research Drive, Durham, NC 27710. Phone: (919) 684-8183. Fax: (919) 681-1678. E-mail: elizabeth.ramsburg{at}duke.edu

{triangledown} Published ahead of print on 25 February 2009.

Clinical and Vaccine Immunology, April 2009, p. 488-498, Vol. 16, No. 4
1071-412X/09/$08.00+0     doi:10.1128/CVI.00451-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»