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 Previous Article

Clinical and Vaccine Immunology, March 2009, p. 433-436, Vol. 16, No. 3
1071-412X/09/$08.00+0     doi:10.1128/CVI.00364-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Simultaneous Detection of Haemophilus influenzae Type b Polysaccharide-Specific Antibodies and Neisseria meningitidis Serogroup A, C, Y, and W-135 Polysaccharide-Specific Antibodies in a Fluorescent-Bead-Based Multiplex Immunoassay {triangledown}

Richarda M. de Voer,1,2* Rutger M. Schepp,1 Florens G. A. Versteegh,3 Fiona R. M. van der Klis,1 and Guy A. M. Berbers1

Laboratory for Infectious Diseases and Screening, National Institute of Public Health and the Environment, Bilthoven, The Netherlands,1 Department of Immunology, University Medical Centre Utrecht, Utrecht, The Netherlands,2 Department of Pediatrics, Groene Hart Ziekenhuis, Gouda, The Netherlands3

Received 3 October 2008/ Returned for modification 4 December 2008/ Accepted 26 December 2008

We expanded the meningococcal serogroup A, C, Y, and W-135 multiplex immunoassay (MIA) to simultaneously detect immunoglobulin type G antibodies directed toward Haemophilus influenzae type b polysaccharide (HibPS). The monoplex HibPS assay was compared to a HibPS-specific competitive enzyme-linked immunosorbent assay and showed a good correlation (R = 0.96). Furthermore, no cross-reactivity between HibPS and the four meningococcal serogroups was detected. This pentaplex meningococcal Hib MIA is a useful tool to investigate serological responses toward different childhood PS vaccines.

* Corresponding author. Mailing address: Laboratory for Infectious Diseases and Screening, National Institute of Public Health and the Environment, Antonie van Leeuwenhoeklaan 1, P.O. Box 1, 3720 BA, Bilthoven, The Netherlands. Phone: 31 30 2742496. Fax: 31 30 2748888. E-mail: Richarda.de.Voer{at}rivm.nl

{triangledown} Published ahead of print on 7 January 2009.

Clinical and Vaccine Immunology, March 2009, p. 433-436, Vol. 16, No. 3
1071-412X/09/$08.00+0     doi:10.1128/CVI.00364-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • de Voer, R. M., van der Klis, F. R. M., Niers, L. E. M., Rijkers, G. T., Berbers, G. A. M. (2009). Absence of Neisseria meningitidis Serogroup C-Specific Antibodies during the First Year of Life in The Netherlands: an Age Group at Risk?. CVI 16: 1521-1523 [Abstract] [Full Text]  


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