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Clinical and Vaccine Immunology, March 2009, p. 372-375, Vol. 16, No. 3
1071-412X/09/$08.00+0     doi:10.1128/CVI.00380-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Acute Parvovirus B19 Infection Frequently Causes False-Positive Results in Epstein-Barr Virus- and Herpes Simplex Virus-Specific Immunoglobulin M Determinations Done on the Liaison Platform{triangledown}

Mario Berth* and Eugene Bosmans

Algemeen Medisch Laboratorium, Immunology Department, Desguinlei 88, 2018 Antwerp, Belgium

Received 17 October 2008/ Returned for modification 24 November 2008/ Accepted 23 December 2008

During an outbreak of parvovirus B19 we collected serum samples from 68 nonpregnant patients in the region of Antwerp (Belgium). Fifty-seven (84%) of the parvovirus B19 immunoglobulin M (IgM)-positive sera had a positive result for Epstein-Barr virus (EBV) IgM by Liaison testing, 61 (90%) had a positive result for herpes simplex virus (HSV) IgM, 20 (29%) samples were positive for cytomegalovirus IgM, and 15 (22%) had a positive result for Borrelia burgdorferi sensu lato IgM. As assay interference was suspected, sera were further investigated by using additional infectious-disease serology tests and by performing various interference elimination procedures. We could show that the EBV IgM and HSV IgM results were false positives due to aspecific IgM reactions with the solid phase. All samples were also analyzed by a modified Liaison EBV IgM assay, based on the addition of polyvinylpyrrolidone and polyvinyl alcohol to the dilution buffer, which partially eliminated this type of assay interference. Although the Liaison is a very convenient, automated immunoassay platform, this study demonstrates the potential for improvement of mainly the EBV IgM and HSV IgM tests.

* Corresponding author. Mailing address: Algemeen Medisch Laboratorium, Immunology Department, Desguinlei 88, 2018 Antwerp, Belgium. Phone: 32 3 248 56 50. Fax: 32 3 216 06 03. E-mail: mario.berth{at}aml-lab.be

{triangledown} Published ahead of print on 30 December 2008.

Clinical and Vaccine Immunology, March 2009, p. 372-375, Vol. 16, No. 3
1071-412X/09/$08.00+0     doi:10.1128/CVI.00380-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Costa, E., Tormo, N., Clari, M. A., Bravo, D., Munoz-Cobo, B., Navarro, D. (2009). Performance of the Epstein-Barr Virus and Herpes Simplex Virus Immunoglobulin M Assays on the Liaison Platform with Sera from Patients Displaying Acute Parvovirus B19 Infection. CVI 16: 1247-1248 [Abstract] [Full Text]  


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