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Clinical and Vaccine Immunology, March 2009, p. 320-322, Vol. 16, No. 3
1071-412X/09/$08.00+0     doi:10.1128/CVI.00409-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Improved Detection of Histoplasma Antigenemia following Dissociation of Immune Complexes{triangledown}

S. Swartzentruber,1 A. LeMonte,1 J. Witt,1 D. Fuller,2 T. Davis,2 C. Hage,2,3 P. Connolly,1 M. Durkin,1 and L. J. Wheat1*

MiraVista Diagnostics, Indianapolis, Indiana,1 Indiana University-Department of Pathology and Laboratory Medicine, Indianapolis, Indiana,2 Roudebush Veterans' Administration Medical Center, Indianapolis, Indiana3

Received 8 November 2008/ Returned for modification 4 December 2008/ Accepted 2 January 2009

The sensitivity for detection of Histoplasma antigen is lower in serum than in urine. In other antigen assays, treatment of serum at 104°C in the presence of EDTA was required for detection of antigenemia. Sensitivity and specificity for detection of Histoplasma antigenemia were examined with or without EDTA heat treatment of the serum using the MVista Histoplasma antigen enzyme immunoassay. A total of 94.6% of serum specimens from patients with AIDS and histoplasmosis that were negative untreated were positive after EDTA-heat treatment. Two-thirds of the negative serum specimens from patients with probable histoplasmosis, based upon clinical suspicion and Histoplasma antigenuria, were positive after heat treatment. Specificity was 99.0% in controls, including healthy subjects and patients in whom histoplasmosis or blastomycosis, were excluded. Precision and reproducibility were good and excellent, respectively. These findings demonstrate improvement in sensitivity without reduction in specificity, precision, or reproducibility after heat-EDTA treatment.

* Corresponding author. Mailing address: MiraVista Diagnostics, 4444 Decatur Blvd., Suite 300, Indianapolis, IN 46241. Phone: (317) 856-2681. Fax: (317) 856-3685. E-mail: jwheat{at}miravistalabs.com

{triangledown} Published ahead of print on 14 January 2009.

Clinical and Vaccine Immunology, March 2009, p. 320-322, Vol. 16, No. 3
1071-412X/09/$08.00+0     doi:10.1128/CVI.00409-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Durkin, M., Estok, L., Hospenthal, D., Crum-Cianflone, N., Swartzentruber, S., Hackett, E., Wheat, L. J. (2009). Detection of Coccidioides Antigenemia following Dissociation of Immune Complexes. CVI 16: 1453-1456 [Abstract] [Full Text]  


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