This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wilson, D. J.
Right arrow Articles by Grohn, Y. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wilson, D. J.
Right arrow Articles by Grohn, Y. T.

 Previous Article  |  Next Article 

Clinical and Vaccine Immunology, February 2009, p. 209-217, Vol. 16, No. 2
1071-412X/09/$08.00+0     doi:10.1128/CVI.00324-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Association of Escherichia coli J5-Specific Serum Antibody Responses with Clinical Mastitis Outcome for J5 Vaccinate and Control Dairy Cattle {triangledown}

David J. Wilson,1* Bonnie A. Mallard,2 Jeanne L. Burton,3,{dagger} Ynte H. Schukken,4 and Yrjo T. Grohn4

Animal, Dairy and Veterinary Sciences, Utah State University, Logan, Utah 84322,1 Department of Pathobiology, University of Guelph, Guelph, Ontario, Canada,2 Department of Animal Science, Michigan State University, East Lansing, Michigan 48824,3 Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York 148504

Received 4 September 2008/ Returned for modification 17 November 2008/ Accepted 24 November 2008

Dairy cattle in two commercial Holstein herds were randomly selected to be vaccinated twice with J5, at approximately 60 days and 28 days before the expected calving date, or to be untreated controls. Based on whether milk production changed following clinical mastitis or whether cows were culled or died within 30 days after onset, 51 mastitis cases were classified as severe or mild. J5-specific antibody responses were evaluated by enzyme-linked immunosorbent assay of all 32 severe and 19 mild cases. The amounts of J5-specific immunoglobulin M (IgM), IgG1, and IgG2 antibodies in sera from the 27 J5 vaccinates were compared with those of the 24 controls. At drying off (before J5 vaccination), all cows had similar amounts of J5-specific antibody. Immediately after calving (approximately 28 days after the second vaccination), J5 vaccinates had significantly higher production of J5-specific IgG1 and IgG2 than controls. When cows were tested following clinical mastitis, none of the three antibody classes differed significantly between the controls and the vaccinates. Vaccinates that contracted Escherichia coli mastitis had 75% less milk loss than controls. The cows that contracted clinical mastitis later in lactation, the unvaccinated controls, and those infected with E. coli had more milk loss following mastitis. The hazards of being culled for all reasons and of being culled for mastitis were significantly lower for J5 vaccinates. Vaccination with J5 was associated with protection against milk production loss and culling following clinical mastitis, and it was also significantly associated with changes in J5-specific IgM, IgG1, and IgG2 antibodies in sera of vaccinated cows.

* Corresponding author. Mailing address: Utah State University, UMC 5700, Logan, UT 84322-5700. Phone: (435) 797-1899. Fax: (435) 797-2805. E-mail: David.Wilson{at}usu.edu

{triangledown} Published ahead of print on 3 December 2008.

{dagger} Deceased.

Clinical and Vaccine Immunology, February 2009, p. 209-217, Vol. 16, No. 2
1071-412X/09/$08.00+0     doi:10.1128/CVI.00324-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»