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Clinical and Vaccine Immunology, November 2009, p. 1675-1686, Vol. 16, No. 11
1071-412X/09/$08.00+0     doi:10.1128/CVI.00224-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Cellular Targeting of Engineered Heterologous Antigens Is a Determinant Factor for Bovine Herpesvirus 4-Based Vaccine Vector Development{triangledown}

Gaetano Donofrio,1* Valentina Franceschi,1 Antonio Capocefalo,1 Simone Taddei,1 Chiara Sartori,1 Sabrina Bonomini,2 Sandro Cavirani,1 Clotilde S. Cabassi,1 and Cesidio F. Flammini1

Department of Animal Health,1 Hematology and Bone Marrow Transplantation Centre, University of Parma, Parma, Italy2

Received 4 June 2009/ Returned for modification 27 July 2009/ Accepted 18 September 2009

In a previous study, an apathogenic strain of bovine herpesvirus 4 (BoHV-4) cloned as a bacterial artificial chromosome and expressing a chimeric peptide (gE2/gD) as a secreted form was described. Recombinant virus-inoculated animals produced antibodies against bovine viral diarrhea virus (BVDV) gE2 and BoHV-1 gD. However, neutralizing antibodies were produced only against BVDV, not against BoHV-1. In the present work a recombinant BoHV-4 expressing a membrane-linked form of gE2/gD chimeric peptide was constructed, and inoculated rabbits produced serum-neutralizing antibodies against both BVDV and BoHV-1. Protein cell sorting and targeting are a very important issue when immunodominant antigens are engineered for recombinant virus vaccine development.

* Corresponding author. Mailing address: Facoltà di Medicina Veterinaria, Dipartimento di Salute Animale, Sezione di Malattie Infettive degli Animali, via del Taglio 10, 43100 Parma, Italy. Phone: 390521032677. Fax: 390521032672. E-mail: gaetano.donofrio{at}unipr.it

{triangledown} Published ahead of print on 30 September 2009.

Clinical and Vaccine Immunology, November 2009, p. 1675-1686, Vol. 16, No. 11
1071-412X/09/$08.00+0     doi:10.1128/CVI.00224-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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