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Clinical and Vaccine Immunology, October 2009, p. 1485-1492, Vol. 16, No. 10
1071-412X/09/$08.00+0     doi:10.1128/CVI.00175-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Immune Responses of Human Immature Dendritic Cells Can Be Modulated by the Recombinant Aspergillus fumigatus Antigen Aspf1{triangledown}

Michael Ok,1 Jean Paul Latgé,2 Carina Baeuerlein,1 Frank Ebel,3 Markus Mezger,1 Max Topp,1 Oliver Kurzai,4 Doreen Killian,1 Markus Kapp,1 Goetz-Ulrich Grigoleit,1 Helga Sennefelder,1 Javier Arroyo,5 Hermann Einsele,1 and Juergen Loeffler1*

Universität Würzburg, Medizinische Klinik & Poliklinik II,1 Institut für Hygiene & Mikrobiologie, Würzburg, Germany,4 Institute Pasteur, Unité des Aspergillus, Paris, France,2 Ludwig-Maximilians-Universität, Max von Pettenkofer Institut, Munich, Germany,3 Universidad Complutense, Departamento Microbiologia II, Madrid, Spain5

Received 19 May 2009/ Returned for modification 16 June 2009/ Accepted 22 July 2009

Invasive aspergillosis is a significant cause of morbidity and mortality in patients after stem cell transplantation, in solid organ transplant recipients, and in patients with hematological malignancies. The interactions between human immature dendritic cells (iDCs) and Aspergillus fumigatus antigens are widely uncharacterized. We analyzed the immune response of iDCs to different recombinant A. fumigatus antigens (Aspf1 and Crf1). One of these antigens, the 18-kDa RNase Aspf1, triggered the increased level of expression of genes encoding proinflammatory cytokines and chemokines, and augmented the activation of NF{kappa}B and the apoptosis of iDCs. Furthermore, by fluorescence microscopy, we could demonstrate that in the first 3 h a major portion of Aspf1 accumulates on the cell surface. Finally, we could show an increased segregation of cytokines and chemokines after the stimulation of iDCs by an Aspf1 deletion mutant strain of A. fumigatus.

* Corresponding author. Mailing address: Medizinische Klinik & Poliklinik II, Josef-Schneider-Str. 2, Würzburg 97080, Germany. Phone: 49 931 201 36412. Fax: 49 931 201 36409. E-mail: loeffler_j{at}klinik.uni-wuerzburg.de

{triangledown} Published ahead of print on 12 August 2009.

Clinical and Vaccine Immunology, October 2009, p. 1485-1492, Vol. 16, No. 10
1071-412X/09/$08.00+0     doi:10.1128/CVI.00175-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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