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Clinical and Vaccine Immunology, October 2009, p. 1453-1456, Vol. 16, No. 10
1071-412X/09/$08.00+0     doi:10.1128/CVI.00227-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Detection of Coccidioides Antigenemia following Dissociation of Immune Complexes {triangledown}

Michelle Durkin,1,2 Lois Estok,3 Duane Hospenthal,4 Nancy Crum-Cianflone,5 Samantha Swartzentruber,1,2 Emily Hackett,1,2 and L. Joseph Wheat1,2*

MiraVista Diagnostics, Indianapolis, Indiana,1 MiraBella Technologies, Indianapolis, Indiana,2 El Rio Special Immunology Associates, Tucson, Arizona,3 Brook Army Medical Center, Fort Sam Houston, Texas,4 Naval Medical Center, San Diego, California5

Received 8 June 2009/ Returned for modification 25 June 2009/ Accepted 3 August 2009

Having reported that pretreatment of serum samples with EDTA at 100°C improved the sensitivity for the detection of Histoplasma antigenemia, we have evaluated this method for the detection of Coccidioides antigenemia. Urine and serum samples from patients with coccidioidomycosis were tested using the MVista Coccidioides enzyme immunoassay, and serum samples with and without EDTA-heat treatment were tested. Antigenemia was detected in 28.6% of patients whose samples were not EDTA-heat treated and in 73.1% of those whose samples were treated. Antigenuria was detected in 50% of patients. Specificity of 100% was obtained in healthy subjects, but cross-reactions were seen in 22.2% of patients with histoplasmosis or blastomycosis. EDTA-heat treatment improves the sensitivity for the detection of Coccidioides antigenemia.

* Corresponding author. Mailing address: MiraVista Diagnostics, 4444 Decatur Blvd., Suite 300, Indianapolis, IN 46241. Phone: (317) 856-2681. Fax: (317) 856-3685. E-mail: jwheat{at}miravistalabs.com

{triangledown} Published ahead of print on 12 August 2009.

Clinical and Vaccine Immunology, October 2009, p. 1453-1456, Vol. 16, No. 10
1071-412X/09/$08.00+0     doi:10.1128/CVI.00227-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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