This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Gowtage-Sequeira, S.
Right arrow Articles by Chambers, M. A.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gowtage-Sequeira, S.
Right arrow Articles by Chambers, M. A.

 Previous Article  |  Next Article 

Clinical and Vaccine Immunology, October 2009, p. 1449-1452, Vol. 16, No. 10
1071-412X/09/$08.00+0     doi:10.1128/CVI.00178-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Evaluation of the CervidTB STAT-PAK for the Detection of Mycobacterium bovis Infection in Wild Deer in Great Britain{triangledown}

S. Gowtage-Sequeira,1* A. Paterson,2 K. P. Lyashchenko,3 S. Lesellier,3 and M. A. Chambers1

TB Research Group, Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency, Weybridge, Surrey KT15 3NB, United Kingdom,1 TB Programme, 6th Floor, Millbank, Nobel House, 17 Smith Square, London SW1P 3JR, United Kingdom,2 Chembio Diagnostic Systems, Inc., Medford, New York3

Received 30 April 2009/ Returned for modification 8 June 2009/ Accepted 29 July 2009

Deer are acknowledged as hosts of Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), and determining the prevalence of infection in deer species is one of the key steps in understanding the epidemiological role played by cervids in the transmission and maintenance of bTB in the United Kingdom. This study evaluated a rapid lateral-flow test for the detection of bTB in samples from wild deer species in the United Kingdom. Fallow deer (Dama dama), roe deer (Capreolus capreolus), and red deer (Cervus elaphus) from areas in Wales, the Cotswolds, and southwestern England were necropsied for a bTB survey. Serum samples from individual deer were tested with the CervidTB STAT-PAK, and the results were evaluated against the culture of M. bovis from tissues (n = 432). Sensitivity and specificity were 85.7% (95% confidence interval [CI], 42.1 to 99.6%) and 94.8% (95% CI, 92.3 to 96.7%), respectively, with an odds ratio of 109.9 (95% CI, 12.7 to 953.6%) for a positive STAT-PAK result among culture-positive deer. The low prevalence of infection (3.8%, n = 860) affected the confidence of the sensitivity estimate of the test, but all culture-positive fallow deer (n = 6) were detected by the test. In addition, antibodies to M. bovis could be detected in poor-quality serum samples. The results suggest that the CervidTB STAT-PAK could be deployed as a field test for further evaluation.

* Corresponding author. Mailing address: TB Research Group, Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, United Kingdom. Phone: 44 1932 357887. Fax: 44 1932 357260. E-mail: s.gowtage{at}vla.defra.gsi.gov.uk

{triangledown} Published ahead of print on 5 August 2009.

Clinical and Vaccine Immunology, October 2009, p. 1449-1452, Vol. 16, No. 10
1071-412X/09/$08.00+0     doi:10.1128/CVI.00178-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»