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Clinical and Vaccine Immunology, January 2009, p. 127-131, Vol. 16, No. 1
1071-412X/09/$08.00+0     doi:10.1128/CVI.00304-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Simple Tests for Rapid Detection of Canine Parvovirus Antigen and Canine Parvovirus-Specific Antibodies{triangledown} ,{dagger}

Shashidhara Y. Marulappa and Sanjay Kapil*

Oklahoma Animal Disease Diagnostic Laboratory (OADDL), Center for Veterinary Health Sciences, Stillwater, Oklahoma 74078

Received 23 August 2008/ Returned for modification 1 October 2008/ Accepted 23 October 2008

Canine parvovirus (CPV) is the number one viral cause of enteritis, morbidity, and mortality in 8-week-old young puppies. We have developed twin assays (slide agglutination test [SAT] for CPV antigen and slide inhibition test [SIT] for CPV antibody) that are sensitive, specific, cost-effective, generic for all genotypes of CPV, and provide instant results for CPV antigen detection in feces and antibody quantification in serum. We found these assays to be useful for routine applications in kennels with large numbers of puppies at risk. The results of these assays are available in 1 min and do not require any special instrumentation. SAT-SIT technology will find applications in rapid screening of samples for other hemagglutinating emerging viruses of animals and humans (influenza virus and severe acute respiratory syndrome coronavirus).

* Corresponding author. Mailing address: Center for Veterinary Health Sciences, OADDL, Farm and Ridge Road, Stillwater, OK 74078. Phone: (405) 744-8809. Fax: (405) 744-8612. E-mail: sanjay.kapil{at}okstate.edu

{triangledown} Published ahead of print on 5 November 2008.

{dagger} Supplemental material for this article may be found at http://cvi.asm.org/.

Clinical and Vaccine Immunology, January 2009, p. 127-131, Vol. 16, No. 1
1071-412X/09/$08.00+0     doi:10.1128/CVI.00304-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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