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Clinical and Vaccine Immunology, January 2009, p. 122-126, Vol. 16, No. 1
1071-412X/09/$08.00+0     doi:10.1128/CVI.00359-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Early Pulmonary Cytokine and Chemokine Responses in Mice Immunized with Three Different Vaccines against Mycobacterium tuberculosis Determined by PCR Array{triangledown} ,{dagger}

JaeHyun Lim,1 Steven C. Derrick,1 Kristopher Kolibab,1 Amy Li Yang,1 Steven Porcelli,2 William R. Jacobs,3 and Sheldon L. Morris1*

Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, United States Food and Drug Administration, Bethesda, Maryland,1 Department of Microbiology and Immunology and Department of Medicine, Albert Einstein College of Medicine, Bronx, New York,2 Department of Microbiology and Immunology, Department of Genetics, and the Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, New York3

Received 30 September 2008/ Returned for modification 26 October 2008/ Accepted 14 November 2008

In this study, the early pulmonary cytokine and chemokine responses in mice immunized with either BCG vaccine, a {Delta}secA2 mutant of Mycobacterium tuberculosis, or a DNA vaccine expressing an ESAT6-antigen 85B fusion protein and then aerogenically challenged with a low dose of M. tuberculosis were evaluated by PCR array. The cellular immune responses at day 10 postchallenge were essentially equivalent in the lungs of mice immunized with either the highly immunogenic BCG vaccine or the {Delta}secA2 M. tuberculosis mutant strain. Specifically, 12 immune biomolecules (including gamma interferon [IFN-{gamma}], interleukin-21 [IL-21], IL-27, IL-17f, CXCL9, CXCL10, and CXCL11) were differentially regulated, relative to the levels for naïve controls, in the lungs of vaccinated mice at this time point. Although the vaccine-related immune responses evoked in mice immunized with the DNA vaccine were relatively limited at 10 days postinfection, upregulation of IFN-{gamma} RNA synthesis as well as increased expression levels of CXCL9, CXCL10, and CXCL11 chemokines were detected.

* Corresponding author. Mailing address: FDA/CBER, Building 29, Room 502, 29 Lincoln Drive, Bethesda, MD 20892. Phone: (301) 496-5978. Fax: (301) 435-5675. E-mail: sheldon.morris{at}fda.hhs.gov

{triangledown} Published ahead of print on 26 November 2008.

{dagger} Supplemental material for this article may be found at http://cvi.asm.org/.

Clinical and Vaccine Immunology, January 2009, p. 122-126, Vol. 16, No. 1
1071-412X/09/$08.00+0     doi:10.1128/CVI.00359-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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