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Clinical and Vaccine Immunology, September 2008, p. 1387-1390, Vol. 15, No. 9
1071-412X/08/$08.00+0     doi:10.1128/CVI.00013-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Measurement of the Humoral Immune Response following an Incident Human Papillomavirus Type 16 or 18 Infection in Young Women by a Pseudovirion-Based Neutralizing Antibody Assay{triangledown}

Jane Steele,1*,{dagger} Stuart Collins,2,{dagger} Kaisheng Wen,1 Gordon Ryan,1 Christothea Constandinou-Williams,1 and Ciaran B. J. Woodman1

CRUK Institute for Cancer Studies,1 CRUK Clinical Trials Unit, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom2

Received 4 January 2008/ Returned for modification 8 May 2008/ Accepted 2 July 2008

We have evaluated a neutralizing antibody assay which uses human papillomavirus (HPV) type 16 (HPV-16) and HPV-18 pseudovirions carrying a secretory alkaline phosphatase reporter gene and which can potentially measure functionally relevant HPV type-specific neutralizing antibodies. The reproducibility of the assay was excellent; for HPV-16, the intra- and interassay kappa values were 0.95 and 0.90, respectively; and for HPV-18, the corresponding values were 0.90 and 0.90. This assay was used to describe the kinetics of the neutralizing antibody response in a cohort of 42 young women who were recruited soon after first intercourse and who first tested positive for HPV-16 DNA or HPV-18 DNA, or both, during follow-up. Most women seroconverted following the first detection of type-specific HPV DNA and remained seropositive until the end of follow-up. Our findings are broadly consistent with those of two other cohort studies which have measured the serological response following an incident infection by using the technically simpler virus-like-particle-based enzyme-linked immunosorbent assay.

* Corresponding author. Mailing address: CRUK Institute for Cancer Studies, University of Birmingham, Vincent Drive, Edgbaston, Birmingham B15 2TT, United Kingdom. Phone: 0121 414 7458. Fax: 0121 414 4486. E-mail: j.c.steele{at}bham.ac.uk

{triangledown} Published ahead of print on 16 July 2008.

{dagger} These authors made equal contributions.

Clinical and Vaccine Immunology, September 2008, p. 1387-1390, Vol. 15, No. 9
1071-412X/08/$08.00+0     doi:10.1128/CVI.00013-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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