This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Djoba Siawaya, J. F.
Right arrow Articles by Walzl, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Djoba Siawaya, J. F.
Right arrow Articles by Walzl, G.

 Previous Article  |  Next Article 

Clinical and Vaccine Immunology, August 2008, p. 1165-1170, Vol. 15, No. 8
1071-412X/08/$08.00+0     doi:10.1128/CVI.00084-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Differential Expression of Interleukin-4 (IL-4) and IL-4{delta}2 mRNA, but Not Transforming Growth Factor Beta (TGF-β), TGF-βRII, Foxp3, Gamma Interferon, T-bet, or GATA-3 mRNA, in Patients with Fast and Slow Responses to Antituberculosis Treatment{triangledown}

Joel Fleury Djoba Siawaya,1* Nchinya Bennedict Bapela,1 Katharina Ronacher,1 Nulda Beyers,2 Paul van Helden,1 and Gerhard Walzl1

Division of Molecular Biology and Human Genetics, MRC Centre for Molecular and Cellular Biology, DST and NRF Centre of Excellence for Biomedical TB Research, Faculty of Health Sciences, Stellenbosch University, Cape Town, South Africa,1 Department of Pediatrics and Desmond Tutu TB Centre, Stellenbosch University, Cape Town, South Africa2

Received 19 February 2008/ Returned for modification 14 April 2008/ Accepted 13 June 2008

This study investigated interleukin-4 (IL-4), IL-4{delta}2, transforming growth factor beta (TGF-β), TGF-βRII, Foxp3, GATA-3, T-bet, and gamma interferon (IFN-{gamma}) transcription in peripheral blood samples of adult pulmonary tuberculosis patients prior to and after 1 week of therapy. Twenty patients with positive results for sputum culture for Mycobacterium tuberculosis were enrolled and treated with directly observed short-course antituberculosis chemotherapy. Early treatment response was assessed. At the end of the intensive phase of treatment (month 2), 12 patients remained sputum culture positive (slow responders) and 8 converted to a negative culture (fast responders). Only the expression levels of IL-4 (4-fold decrease) and IL-4{delta}2 (32-fold increase) changed significantly during the first week of therapy in the 20 patients. No baseline differences were present between the responder groups, but fast responders had significantly higher IL-4 transcripts than slow responders at week 1. Fast responders showed a 19-fold upregulation and slow responders a 47-fold upregulation of IL-4{delta}2 at week 1. Only slow responders also showed a significant decrease in IL-4 expression at week 1. There were no significant differences in expression of TGF-β, TGF-βRII, Foxp3, IFN-{gamma}, and GATA-3 between the groups. These data show that differential IL-4-related gene expression in the early stage of antituberculosis treatment accompanies differential treatment responses and may hold promise as a marker for treatment effect.

* Corresponding author. Mailing address: Division of Molecular Biology and Human Genetics, MRC Centre for Molecular and Cellular Biology, DST and NRF Centre of Excellence for Biomedical TB Research, Faculty of Health Sciences, Stellenbosch University, Cape Town 7505, South Africa. Phone: 00 27 21 938 9402. Fax: 00 27 21 938 9476. E-mail: joe{at}sun.ac.za

{triangledown} Published ahead of print on 25 June 2008.

Clinical and Vaccine Immunology, August 2008, p. 1165-1170, Vol. 15, No. 8
1071-412X/08/$08.00+0     doi:10.1128/CVI.00084-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mdivani, N., Li, H., Akhalaia, M., Gegia, M., Goginashvili, L., Kernodle, D. S., Khechinashvili, G., Tang, Y.-W. (2009). Monitoring Therapeutic Efficacy by Real-Time Detection of Mycobacterium tuberculosis mRNA in Sputum. Clin. Chem. 55: 1694-1700 [Abstract] [Full Text]  
  • Thacker, T. C., Palmer, M. V., Waters, W. R. (2009). T-Cell mRNA Expression in Response to Mycobacterium bovis BCG Vaccination and Mycobacterium bovis Infection of White-Tailed Deer. CVI 16: 1139-1145 [Abstract] [Full Text]  
  • Hernandez-Pando, R., Aguilar, D., Orozco, H., Cortez, Y., Brunet, L. R., Rook, G. A. (2008). Orally Administered Mycobacterium vaccae Modulates Expression of Immunoregulatory Molecules in BALB/c Mice with Pulmonary Tuberculosis. CVI 15: 1730-1736 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»