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Clinical and Vaccine Immunology, July 2008, p. 1141-1143, Vol. 15, No. 7
1071-412X/08/$08.00+0     doi:10.1128/CVI.00430-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Effects of Delays in Peripheral Blood Processing, Including Cryopreservation, on Detection of CD31 Expression on Naïve CD4 T Cells{triangledown}

Jeanette Higgins,1 Julia A. Metcalf,2 Randy A. Stevens,1 Michael Baseler,1 Michael Proschan,2 H. Clifford Lane,2 and Irini Sereti2*

AIDS Monitoring Laboratory, Clinical Services Program, SAIC-Frederick, Inc., NCI—Frederick, Frederick, Maryland 21702,1 Clinical and Molecular Retroviral Section, Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 208922

Received 31 October 2007/ Returned for modification 25 December 2007/ Accepted 21 April 2008

Delayed processing of peripheral blood or peripheral blood mononuclear cell isolation and cryopreservation can lead to the detection of somewhat higher levels of CD31 expression on naïve CD4 T cells by flow cytometry. These observations should be considered in the planning of multicenter clinical trials and in the interpretation of the results of functional studies.

* Corresponding author. Mailing address: Clinical and Molecular Retrovirology Section, Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bldg. 10, Room 11B-04, 10 Center Drive MSC 1876, Bethesda, MD 20892. Phone: (301) 496-5533. Fax: (301) 402-0070. E-mail: isereti{at}niaid.nih.gov

{triangledown} Published ahead of print on 30 April 2008.

Clinical and Vaccine Immunology, July 2008, p. 1141-1143, Vol. 15, No. 7
1071-412X/08/$08.00+0     doi:10.1128/CVI.00430-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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