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Clinical and Vaccine Immunology, July 2008, p. 1138-1140, Vol. 15, No. 7
1071-412X/08/$08.00+0     doi:10.1128/CVI.00088-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Development and Characterization of an Equine Infectious Anemia Virus Env-Pseudotyped Reporter Virus{triangledown}

R. L. Tallmadge,1 M. A. Brindley,2 J. Salmans,1 R. H. Mealey,1 W. Maury,2 and S. Carpenter1*

Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164,1 Department of Microbiology, University of Iowa, Iowa City, Iowa 522422

Received 12 March 2008/ Returned for modification 3 April 2008/ Accepted 18 April 2008

We developed a replication-defective reporter virus pseudotyped with the envelope glycoprotein of equine infectious anemia virus (EIAV). The in vitro host range and neutralization phenotype of EIAV Env-pseudotyped virus were similar to those of replication-competent virus. An EIAV Env pseudovirus will improve antigenic characterization of viral variants and evaluation of lentivirus vaccines.

* Corresponding author. Mailing address: Department of Veterinary Microbiology and Pathology, Washington State University, P.O. Box 647040, Pullman, WA 99163. Phone: (509) 335-6043. Fax: (509) 335-8529. E-mail: scarp{at}vetmed.wsu.edu

{triangledown} Published ahead of print on 30 April 2008.

Clinical and Vaccine Immunology, July 2008, p. 1138-1140, Vol. 15, No. 7
1071-412X/08/$08.00+0     doi:10.1128/CVI.00088-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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