This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Berth, M.
Right arrow Articles by Bosmans, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Berth, M.
Right arrow Articles by Bosmans, E.

 Previous Article  |  Next Article 

Clinical and Vaccine Immunology, May 2008, p. 891-892, Vol. 15, No. 5
1071-412X/08/$08.00+0     doi:10.1128/CVI.00012-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Prevention of Assay Interference in Infectious-Disease Serology Tests Done on the Liaison Platform{triangledown}

Mario Berth* and Eugene Bosmans

Algemeen Medisch Laboratorium, Immunology Department, Desguinlei 88, 2018 Antwerpen, Belgium

Received 14 January 2008/ Returned for modification 13 February 2008/ Accepted 3 March 2008

Immunoassay interference causing unexpected reactive results in magnetic-microparticle-based assays was detected. A systematic evaluation of Liaison Epstein-Barr virus immunoglobulin M showed that 5% of the positive results (0.4% of tested samples) could be explained by such interference. Adding chemical blocking reagents (polyvinylpyrrolidone and polyvinyl alcohol) to the assay buffers partially prevented this phenomenon.

* Corresponding author. Mailing address: Algemeen Medisch Laboratorium, Immunology Department, Desguinlei 88, 2018 Antwerpen, Belgium. Phone: 32 3 248 56 50. Fax: 32 3 216 06 03. E-mail: mario.berth{at}aml-lab.be

{triangledown} Published ahead of print on 12 March 2008.

Clinical and Vaccine Immunology, May 2008, p. 891-892, Vol. 15, No. 5
1071-412X/08/$08.00+0     doi:10.1128/CVI.00012-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Costa, E., Tormo, N., Clari, M. A., Bravo, D., Munoz-Cobo, B., Navarro, D. (2009). Performance of the Epstein-Barr Virus and Herpes Simplex Virus Immunoglobulin M Assays on the Liaison Platform with Sera from Patients Displaying Acute Parvovirus B19 Infection. CVI 16: 1247-1248 [Abstract] [Full Text]  
  • Berth, M., Bosmans, E. (2009). Acute Parvovirus B19 Infection Frequently Causes False-Positive Results in Epstein-Barr Virus- and Herpes Simplex Virus-Specific Immunoglobulin M Determinations Done on the Liaison Platform. CVI 16: 372-375 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»