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Clinical and Vaccine Immunology, May 2008, p. 737-743, Vol. 15, No. 5
1071-412X/08/$08.00+0     doi:10.1128/CVI.00254-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

A Neutralizing Antibody to the A Chain of Abrin Inhibits Abrin Toxicity both In Vitro and In Vivo{triangledown}

Kalpana Surendranath{dagger} and Anjali A. Karande*

Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India

Received 18 June 2007/ Returned for modification 7 August 2007/ Accepted 11 March 2008

Plant ribosome-inactivating proteins (RIPs) are RNA N-glycosidases that inhibit protein synthesis in cells. Abrin, a type II RIP, is an AB type toxin, which is one of the most lethal types of toxin known. The B chain facilitates the entry of the molecule into the cell, whereas the A chain exerts the toxic effect. We have generated hybridomas secreting antibodies of the immunoglobulin G class specific to the recombinant A chain of abrin. One monoclonal antibody, namely, D6F10, rescued cells from abrin toxicity. Importantly, the antibody also protected mice from lethal doses of the toxin. The neutralizing effect of the antibody was shown to be due to interference with abrin attachment to the cell surface.

* Corresponding author. Mailing address: Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India. Phone: 91 80 2293 2306. Fax: 91 80 2360 0814. E-mail: anjali{at}biochem.iisc.ernet.in

{triangledown} Published ahead of print on 19 March 2008.

{dagger} Present address: Institute of Molecular Cancer Research, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.

Clinical and Vaccine Immunology, May 2008, p. 737-743, Vol. 15, No. 5
1071-412X/08/$08.00+0     doi:10.1128/CVI.00254-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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