This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ohya, K.
Right arrow Articles by Fukushi, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ohya, K.
Right arrow Articles by Fukushi, H.

 Previous Article  |  Next Article 

Clinical and Vaccine Immunology, October 2008, p. 1606-1615, Vol. 15, No. 10
1071-412X/08/$08.00+0     doi:10.1128/CVI.00134-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Chlamydophila felis CF0218 Is a Novel TMH Family Protein with Potential as a Diagnostic Antigen for Diagnosis of C. felis Infection{triangledown} ,{dagger}

Kenji Ohya,1 Yu Takahara,1 Etsuko Kuroda,1 Saori Koyasu,1 Shigeyuki Hagiwara,2 Maki Sakamoto,2 Mitsuaki Hisaka,2 Kazuko Morizane,2 Shinryou Ishiguro,2 Tsuyoshi Yamaguchi,1,3 and Hideto Fukushi1,3*

Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences,1 Department of Applied Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193,3 Advanced Technology Development Center, Kyoritsu Seiyaku Corporation, Tsukuba 300-1252, Japan2

Received 16 April 2008/ Returned for modification 28 May 2008/ Accepted 21 August 2008

Chlamydophila felis is a causative agent of acute and chronic conjunctivitis and pneumonia in cats (feline chlamydiosis). Also, C. felis is a suspected zoonotic agent of such diseases as non-Chlamydia trachomatis conjunctivitis in humans, although this is controversial. At present, there is no serodiagnostic system that specifically detects C. felis infection conveniently. Current systems use antigens such as lipopolysaccharide that cross-react with all chlamydia species. In addition, it is difficult to distinguish between cats that are vaccinated with the commercial vaccine against C. felis and cats that are infected with C. felis. Here, we describe a new candidate diagnostic antigen for diagnosis of C. felis infection, CF0218, that was obtained by screening a genomic expression library of C. felis Fe/C-56 with C. felis-immunized serum. CF0218 was a putative transmembrane head (TMH) family protein with bilobed hydrophobic motifs at its N terminus, and orthologues of CF0218 were not found in the Chlamydophila pneumoniae or Chlamydia trachomatis genomes. The recombinant CF0218 was not recognized by antiserum against C. trachomatis, suggesting that CF0218 is C. felis specific. CF0218 transcription during the course of C. felis infection was confirmed by reverse transcription-PCR. By indirect immunofluorescence analysis, CF0218 was colocalized with the C. felis-formed inclusion bodies in the infected cells. The antibody response against CF0218 was elevated following C. felis infection but not by vaccination in experimentally vaccinated and infected cats. These results suggest that CF0218, a novel TMH family protein of C. felis, possesses potential as a C. felis infection-specific diagnostic antigen.

* Corresponding author. Mailing address: Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan. Phone: 81-58-293-2946. Fax: 81-58-293-2946. E-mail: hfukushi{at}gifu-u.ac.jp

{triangledown} Published ahead of print on 3 September 2008.

{dagger} Supplemental material for this article may be found at http://cvi.asm.org/.

Clinical and Vaccine Immunology, October 2008, p. 1606-1615, Vol. 15, No. 10
1071-412X/08/$08.00+0     doi:10.1128/CVI.00134-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»