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Clinical and Vaccine Immunology, October 2008, p. 1572-1579, Vol. 15, No. 10
1071-412X/08/$08.00+0     doi:10.1128/CVI.00156-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Characterization of a Recombinant Newcastle Disease Virus Vaccine Strain{triangledown}

Sun-Hee Cho,1,{dagger} Hyuk-Joon Kwon,1* Tae-Eun Kim,1 Jae-Hong Kim,1 Han-Sang Yoo,1 Man-Hoon Park,2 Young-Ho Park,3 and Sun-Joong Kim1,{dagger}

Zoonotic Disease Institute (ZooDI), College of Veterinary Medicine and BK21 for Veterinary Science, Seoul National University, Seoul,1 Mogam Biotechnology Research Institute, Yong-In, Gyeonggi-Do,2 KBNP Inc., Yesan, Choongcheongnam-do, Korea3

Received 2 May 2008/ Returned for modification 13 June 2008/ Accepted 21 August 2008

A recombinant La Sota strain (KBNP-C4152R2L) in which fusion (F) and hemagglutinin-neuraminidase (HN) genes were replaced with those of a contemporary genotype VIId virus, KBNP-4152, has been developed. To attenuate the virulence of the recombinant strain, the F cleavage motif was mutated from 112RRQKR116 to 112GRQAR116, and to reduce pathogenic instability, a codon which does not allow changes to basic amino acids by single point mutation was inserted at codon 115. In addition a six-nucleotide sequence was inserted into the intergenic region between matrix protein and F genes for attenuation without breaking the "rule-of-six." The HN protein length was increased from 571 to 577 as a marker. Serological tests revealed that the antigenicity of KBNP-C4152R2L was similar to that of KBNP-4152 but distinct from that of the La Sota strain. KBNP-C4152R2L was avirulent (intracerebral pathogenicity index, 0.0; mean death time, >168 h) and stable in pathogenicity through in vivo passages. The killed oil emulsion of and live KBNP-C4152R2L were completely protective against mortality and egg drop caused by virulent strains, and KBNP-C4152R2L was applicable to in ovo vaccination. Therefore, KBNP-C4152R2L is a promising vaccine strain and viral vector in terms of antigenicity, productivity, safety, and pathogenic stability.

* Corresponding author. Mailing address: 151-742, Laboratory of Influenza Virus, Zoonotic Disease Institute (ZooDI), Seoul National University, San 56-1, Shillim-Dong, Gwanak-Gu, Seoul, Korea. Phone: 82-2-880-1288. Fax: 82-2-880-1233. E-mail: kwonhj01{at}snu.ac.kr

{triangledown} Published ahead of print on 3 September 2008.

{dagger} Present address: 441-853 BioPOA Co., Suwon, Gyeonggi-Do, Korea.

Clinical and Vaccine Immunology, October 2008, p. 1572-1579, Vol. 15, No. 10
1071-412X/08/$08.00+0     doi:10.1128/CVI.00156-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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