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Clinical and Vaccine Immunology, October 2008, p. 1529-1535, Vol. 15, No. 10
1071-412X/08/$08.00+0     doi:10.1128/CVI.00181-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Development of Antibodies to PspA Families 1 and 2 in Children after Exposure to Streptococcus pneumoniae{triangledown}

Merit M. Melin,1* Susan K. Hollingshead,2 David E. Briles,2 Mika I. Lahdenkari,1 Terhi M. Kilpi,1 and Helena M. Käyhty1

National Public Health Institute, Helsinki, Finland,1 University of Alabama at Birmingham, Birmingham, Alabama2

Received 15 May 2008/ Returned for modification 27 June 2008/ Accepted 13 August 2008

Pneumococcal surface protein A (PspA) is an important virulence factor of Streptococcus pneumoniae. PspA exists as two major families, which include variable but serologically cross-reactive proteins. Previous studies with a family 1 PspA antigen suggested that children develop low concentrations of anti-PspA after pneumococcal carriage or infection. In this study, antibody to PspA families 1 and 2 was measured by an enzyme immunoassay of the serum and saliva of children with a history of culture-proven pneumococcal colonization and/or acute otitis media and in the serum and saliva of adults. The PspA families of the pneumococcal strains isolated from children were determined. The majority of the children had high serum and salivary anti-PspA concentrations to the PspA family they had encountered and low concentrations to the other, whereas adults had high antibody concentrations to both PspA families, both in serum and in saliva. The results suggest that children have a relatively family-specific antibody response to the PspA family they have been exposed to and that any PspA vaccine for children should contain members of both major PspA families.

* Corresponding author. Mailing address: National Public Health Institute (KTL), Department of Vaccines, Mannerheimintie 166, 00300 Helsinki, Finland. Phone: 358-9-4744 8903. Fax: 358-9-4744 8599. E-mail: merit.melin{at}ktl.fi

{triangledown} Published ahead of print on 27 August 2008.

Clinical and Vaccine Immunology, October 2008, p. 1529-1535, Vol. 15, No. 10
1071-412X/08/$08.00+0     doi:10.1128/CVI.00181-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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