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Clinical and Vaccine Immunology, October 2008, p. 1497-1504, Vol. 15, No. 10
1071-412X/08/$08.00+0     doi:10.1128/CVI.00166-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Primary CD8+ T-Cell Response to Soluble Ovalbumin Is Improved by Chloroquine Treatment In Vivo{triangledown}

Bruno Garulli,1,2 Maria G. Stillitano,1 Vincenzo Barnaba,3 and Maria R. Castrucci1*

Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, 00161 Rome,1 Department of Cellular and Developmental Biology, University of Rome La Sapienza, Rome,2 Fondazione Andrea Cesalpino, Department of Internal Medicine, University of Rome La Sapienza, Rome, Italy3

Received 12 May 2008/ Returned for modification 23 June 2008/ Accepted 15 August 2008

The efficiency of cross-presentation of exogenous antigens by dendritic cells (DCs) would seem to be related to the level of antigen escape from massive degradation mediated by lysosomal proteases in an acidic environment. Here, we demonstrate that a short course of treatment with chloroquine in mice during primary immunization with soluble antigens improved the cross-priming of naïve CD8+ T lymphocytes in vivo. More specifically, priming of chloroquine-treated mice with soluble ovalbumin (OVA), OVA associated with alum, or OVA pulsed on DCs was more effective in inducing OVA-specific CD8+ T lymphocytes than was priming of untreated mice. We conclude that chloroquine treatment improves the cross-presentation capacity of DCs and thus the size of effector and memory CD8+ T cells during vaccination.

* Corresponding author. Mailing address: Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena, 299-00161 Rome, Italy. Phone: (3906) 49903259. Fax: (3906) 49902082. E-mail: maria.castrucci{at}iss.it

{triangledown} Published ahead of print on 27 August 2008.

Clinical and Vaccine Immunology, October 2008, p. 1497-1504, Vol. 15, No. 10
1071-412X/08/$08.00+0     doi:10.1128/CVI.00166-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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