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Clinical and Vaccine Immunology, January 2008, p. 164-167, Vol. 15, No. 1
1071-412X/08/$08.00+0     doi:10.1128/CVI.00201-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Use of Sera from Humans and Dolphins with Lacaziosis and Sera from Experimentally Infected Mice for Western Blot Analyses of Lacazia loboi Antigens{triangledown}

Leonel Mendoza,1* Andréa F. F. Belone,2 Raquel Vilela,1 Manuela Rehtanz,3 Gregory D. Bossart,4 John S. Reif,5 Patricia A. Fair,6 Wendy N. Durden,7 Judy St. Leger,8 Luiz R. Travassos,9 and Patricia S. Rosa2

Biomedical Laboratory Diagnostics Program, Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824-1031,1 Instituto Lauro de Souza Lima, Bauru, São Paulo, Brazil,2 New York University, Department of Basic Sciences, Infectious Diseases, New York, New York 10010,3 Harbor Branch Oceanographic Institution, Center for Coastal Research, Marine Mammal Research and Conservation, Fort Pierce, Florida 34946,4 Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, Colorado 80523,5 National Oceanic and Atmospheric Administration/National Ocean Service/Center for Coastal Environmental Health and Biomolecular Research, Charleston, South Carolina 29412,6 Hubbs-SeaWorld Research Institute, Orlando, Florida 32821-8043,7 Department of Pathology, SeaWorld, San Diego, California 92109,8 Department of Microbiology, Immunology and Parasitology, Universidade Federal de São Paulo, São Paulo, Brazil9

Received 14 May 2007/ Returned for modification 5 August 2007/ Accepted 16 October 2007

Antibodies in the sera of patients with lacaziosis recognized an ~193-kDa antigen and other Lacazia loboi antigens. Paracoccidioides brasiliensis gp43 antigen was detected by all evaluated sera, but they failed to detect a protein with the same molecular mass in L. loboi extracts. This study is the first to examine the humoral response to L. loboi antigens by using multiple host sera.

* Corresponding author. Mailing address: Biomedical Laboratory Diagnostics Program, Michigan State University, 322 North Kedzie Hall, East Lansing, MI 48824-1031. Phone: (517) 353-7800. Fax: (517) 432-2006. E-mail: mendoza9{at}msu.edu

{triangledown} Published ahead of print on 24 October 2007.

Clinical and Vaccine Immunology, January 2008, p. 164-167, Vol. 15, No. 1
1071-412X/08/$08.00+0     doi:10.1128/CVI.00201-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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