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Clinical and Vaccine Immunology, January 2008, p. 147-153, Vol. 15, No. 1
1071-412X/08/$08.00+0     doi:10.1128/CVI.00363-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Usefulness of Four Different Echinococcus granulosus Recombinant Antigens for Serodiagnosis of Unilocular Hydatid Disease (UHD) and Postsurgical Follow-Up of Patients Treated for UHD

IRNASA, CSIC, Cordel de Merinas, 50, 37008 Salamanca, Spain,¹ Laboratorio de Parasitología, CIETUS, Facultad de Farmacia, Universidad de Salamanca, Campo Charro, s/n, 37007 Salamanca, Spain,² Departamento de Estadística, Facultad de Biología, Universidad de Salamanca, Campo Charro, s/n, 37007 Salamanca, Spain,³ Hospital Universitario de Valladolid, Ramón y Cajal, 3, 47003 Valladolid, Spain⁴

Received 29 August 2007/ Returned for modification 2 October 2007/ Accepted 23 October 2007

Four different recombinant antigens derived from Echinococcus granulosus, designated B1t, B2t, E14t, and C317, were tested with enzyme-linked immunosorbent assays (ELISAs) for the detection of specific immunoglobulin G (IgG) in patients with unilocular hydatid disease (UHD). The results were compared to those obtained with hydatid fluid and were subjected to receiver operator characteristic analysis. The diagnostic performance of the above-listed proteins was defined with respect to their specificity, sensitivity, and predictive values (PV); the influence of cyst location; and usefulness in the follow-up of surgical treatment for UHD and in the determination of whether or not patients have been surgically cured of UHD. The best diagnostic results were obtained with the anti-B2t IgG ELISA, with 91.2% sensitivity, 93% specificity, and high positive and negative PV (89.4 and 94.2, respectively). In addition, this diagnostic tool proved to be useful for the follow-up of surgically treated UHD patients. The anti-B2t IgG ELISA may find an application in the serodiagnosis of UHD in clinical laboratories.

Published ahead of print on 7 November 2007.