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 Previous Article

Clinical and Vaccine Immunology, June 2007, p. 811-812, Vol. 14, No. 6
1071-412X/07/$08.00+0     doi:10.1128/CVI.00011-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Use of Protein-Specific Monoclonal Antibody-Based Latex Agglutination for Rapid Diagnosis of Burkholderia pseudomallei Infection in Patients with Community-Acquired Septicemia{triangledown}

Pattama Ekpo,1* Utane Rungpanich,1 Supinya Pongsunk,2 Pimjai Naigowit,3 and Vimon Petkanchanapong3

Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok,1 Department of Microbiology, Faculty of Medicine, Srinakarinwirot University, Bangkok,2 National Institute of Health, Department of Medical Services, Ministry of Public Health, Nonthaburi, Thailand3

Received 13 December 2006/ Returned for modification 16 February 2007/ Accepted 28 March 2007

A latex agglutination test employing monoclonal antibody specific to a 30-kDa protein of Burkholderia pseudomallei was used to detect the organisms in blood culture specimens from 1,139 patients with community-acquired septicemia. The sensitivity, specificity, and positive and negative predictive values of the test were 96.75%, 99.61%, 96.75%, and 99.61%, respectively.

* Corresponding author. Mailing address: Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. Phone: 662-418-0569. Fax: 662-418-1636. E-mail: sipep{at}mahidol.ac.th

{triangledown} Published ahead of print on 11 April 2007.

Clinical and Vaccine Immunology, June 2007, p. 811-812, Vol. 14, No. 6
1071-412X/07/$08.00+0     doi:10.1128/CVI.00011-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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