This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wilson, D. J.
Right arrow Articles by Gröhn, Y. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wilson, D. J.
Right arrow Articles by Gröhn, Y. T.

 Previous Article  |  Next Article 

Clinical and Vaccine Immunology, June 2007, p. 693-699, Vol. 14, No. 6
1071-412X/07/$08.00+0     doi:10.1128/CVI.00104-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Milk and Serum J5-Specific Antibody Responses, Milk Production Change, and Clinical Effects following Intramammary Escherichia coli Challenge for J5 Vaccinate and Control Cows{triangledown}

David J. Wilson,1* Bonnie A. Mallard,2 Jeanne L. Burton,3 Ynte H. Schukken,1 and Yrjo T. Gröhn1

Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York 14850,1 Department of Pathobiology, University of Guelph, Guelph, Ontario, Canada,2 Department of Animal Science, Michigan State University, East Lansing, Michigan 488243

Received 1 March 2007/ Returned for modification 12 April 2007/ Accepted 16 April 2007

Holstein dairy cows (four J5 vaccinates and four controls) selected for no recorded intramammary disease and low somatic cell count (SCC) during the previous lactation were challenged by intramammary infusion of Escherichia coli. Vaccination with J5 was at 8 weeks and again 4 weeks before the anticipated calving date. Cows were challenged at 8 to 16 days in milk (DIM). Shedding of E. coli in milk was significantly higher among controls than vaccinates (no shedding) from 6 h to 21 h postchallenge. From 21 h to 132 h postchallenge, SCC in challenged quarters of controls (5,429,000/ml) was significantly higher than that of vaccinates (490,000/ml). On the day after challenge, milk production in control cows was 8 kg less, while vaccinates gained 0.5 kg, a significant difference. In serum immediately prior to challenge, J5-specific immunoglobulin G1 (IgG1) was significantly higher, IgG2 was nearly significantly higher, and IgM was the same in J5 vaccinates relative to controls. Vaccinates had proportionally more IgG2 in serum postcalving and in the first 12 h following challenge and less IgG2 in milk 24 h after challenge than the controls, approaching statistical significance. The ratio of J5-specific IgG1 and IgG2 combined compared to IgM was significantly higher in vaccinates than in controls in prechallenge serum (ratios of 15.8 and 3.2, respectively) and milk (5.0 and 1.3, respectively). Cows with higher IgM titers in milk 12 h postchallenge produced significantly less milk. Vaccination with J5 was significantly associated with higher production of J5-specific IgG1 and IgG2 in early lactation, reduced SCC, faster clearance of E. coli from milk, and less milk production loss following intramammary challenge.

* Corresponding author. Mailing address: Utah State University, UMC 5700, Logan, UT 84322-5700. Phone: (435) 797-1899. Fax: (435) 797-2805. E-mail: David.Wilson{at}usu.edu

{triangledown} Published ahead of print on 25 April 2007.

Clinical and Vaccine Immunology, June 2007, p. 693-699, Vol. 14, No. 6
1071-412X/07/$08.00+0     doi:10.1128/CVI.00104-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schukken, Y. H., Hertl, J., Bar, D., Bennett, G. J., Gonzalez, R. N., Rauch, B. J., Santisteban, C., Schulte, H. F., Tauer, L., Welcome, F. L., Grohn, Y. T. (2009). Effects of repeated gram-positive and gram-negative clinical mastitis episodes on milk yield loss in Holstein dairy cows. J DAIRY SCI 92: 3091-3105 [Abstract] [Full Text]  
  • Wilson, D. J., Mallard, B. A., Burton, J. L., Schukken, Y. H., Grohn, Y. T. (2009). Association of Escherichia coli J5-Specific Serum Antibody Responses with Clinical Mastitis Outcome for J5 Vaccinate and Control Dairy Cattle. CVI 16: 209-217 [Abstract] [Full Text]  
  • Bar, D., Tauer, L. W., Bennett, G., Gonzalez, R. N., Hertl, J. A., Schukken, Y. H., Schulte, H. F., Welcome, F. L., Grohn, Y. T. (2008). The Cost of Generic Clinical Mastitis in Dairy Cows as Estimated by Using Dynamic Programming. J DAIRY SCI 91: 2205-2214 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»