This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ohara, E.
Right arrow Articles by Sugiura, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ohara, E.
Right arrow Articles by Sugiura, T.

 Previous Article  |  Next Article 

Clinical and Vaccine Immunology, June 2007, p. 678-684, Vol. 14, No. 6
1071-412X/07/$08.00+0     doi:10.1128/CVI.00426-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Exocytosis of Neutrophil Formyl Peptide Receptor-Like 1 (fPRL1) Results in Downregulation of Cytoplasmic fPRL1 in Patients with Purulent Dermatitis{triangledown}

Eiji Ohara,1,{dagger} Yoshitaka Kumon,1,{dagger}* Toshihiro Kobayashi,2 Hiroaki Takeuchi,1 and Tetsuro Sugiura1

Department of Laboratory Medicine,1 Department of Anatomy and Cell Biology, Kochi Medical School, Kochi University, Kohasu Okoh-Cho, Nankoku, Kochi 783-8505, Japan2

Received 9 October 2006/ Returned for modification 20 January 2007/ Accepted 12 April 2007

N-Formyl peptide receptor-like 1 (fPRL1) is a member of the chemoattractant subfamily of G protein-coupled receptors and plays a key role in inflammation via chemotaxis and the regulation of mediator release from leukocytes. Activated fPRL1 has recently been shown to induce a complicated pattern of cellular signaling in vitro, but the details of the regulation and alteration of leukocyte cellular fPRL1 during inflammation in vivo remain unclear. To clarify the alteration of neutrophil fPRL1 during inflammation in vivo, the immunohistochemical staining of neutrophil fPRL1 in samples from patients with purulent dermatitis was performed. The in vitro morphological alteration of neutrophil fPRL1 on cellular membranes by stimulation with N-formylmethionyl-leucyl-phenylalanine (fMLP) was also examined. Both the cytoplasm and the cellular membranes of blood neutrophils stained strongly for fPRL1. On the other hand, the cellular membranes of neutrophils in dermatitis tissue stained strongly for fPRL1 but the cytoplasm stained weakly. The enhancement of neutrophil fPRL1 on cellular membranes by stimulation with fMLP indicates the exocytosis of neutrophil fPRL1-containing granules. In conclusion, we for the first time confirmed the alteration of neutrophil fPRL1 in clinical cases of purulent dermatitis. Cytoplasm that was weakly stained and cellular membranes that were well stained for fPRL1 were considered to be distinctive features of activated neutrophils in purulent dermatitis tissue.

* Corresponding author. Mailing address: Department of Laboratory Medicine, Kochi Medical School, Kochi University, Kohasu Okoh-Cho, Nankoku, Kochi 783-8505, Japan. Phone: 81-88-880-2427. Fax: 81-88-880-2428. E-mail: kumony{at}kochi-u.ac.jp

{triangledown} Published ahead of print on 25 April 2007.

{dagger} E. Ohara and Y. Kumon contributed equally to this work.

Clinical and Vaccine Immunology, June 2007, p. 678-684, Vol. 14, No. 6
1071-412X/07/$08.00+0     doi:10.1128/CVI.00426-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»