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Clinical and Vaccine Immunology, March 2007, p. 323-327, Vol. 14, No. 3
1071-412X/07/$08.00+0     doi:10.1128/CVI.00302-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Use of an Inhibition Enzyme-Linked Immunosorbent Assay for Quantification of Capsular Polysaccharide or Proteins in Vaccines{triangledown}

Thomas J. Inzana* and Anna Champion

Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061

Received 25 August 2006/ Returned for modification 12 October 2006/ Accepted 12 January 2007

An inhibition enzyme-linked immunosorbent assay (ELISA) is described for quantification of capsular polysaccharide or proteins in vaccines and other samples containing whole cells or extracts of Actinobacillus pleuropneumoniae. The assay can be used to quantify any antigen that can be purified and for which highly specific antibodies are not available. The assay can be carried out by any laboratory capable of performing an ELISA.

* Corresponding author. Mailing address: College of Veterinary Medicine, 1800 Kraft Dr., Suite 200, CMMID, Virginia Tech, 0491, Blacksburg, VA 24061. Phone: (540) 231-4692. Fax: (540) 231-5553. E-mail: tinzana{at}vt.edu.

{triangledown} Published ahead of print on 31 January 2007.

Clinical and Vaccine Immunology, March 2007, p. 323-327, Vol. 14, No. 3
1071-412X/07/$08.00+0     doi:10.1128/CVI.00302-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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Copyright © 2007 by the American Society for Microbiology. All rights reserved.