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Clinical and Vaccine Immunology, March 2007, p. 244-249, Vol. 14, No. 3
1071-412X/07/$08.00+0     doi:10.1128/CVI.00430-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Use of Mycelial-Phase Sporothrix schenckii Exoantigens in an Enzyme-Linked Immunosorbent Assay for Diagnosis of Sporotrichosis by Antibody Detection{triangledown}

Rodrigo Almeida-Paes,1,3 Monique A. Pimenta,1 Claudia Vera Pizzini,1 Paulo Cezar F. Monteiro,1 José Mauro Peralta,2 Joshua D. Nosanchuk,3,4 and Rosely Maria Zancopé-Oliveira1*

Serviço de Micologia, Instituto de Pesquisa Clínica Evandro Chagas, Fiocruz, Rio de Janeiro, Brazil,1 Departamento de Imunologia, Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil,2 Division of Infectious Diseases, Department of Medicine,3 Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York4

Received 13 November 2006/ Returned for modification 14 December 2006/ Accepted 2 January 2007

An enzyme-linked immunosorbent assay (ELISA) was developed for specific antibody detection in serum specimens of patients with sporotrichosis. The assay was made with mycelial-phase Sporothrix schenckii exoantigens and was tested against 90 sera from patients with different clinical forms of sporotrichosis. Potential cross-reactions were analyzed with 72 heterologous sera from patients with paracoccidioidomycosis, cryptococcosis, aspergillosis, histoplasmosis, tuberculosis, and American tegumentary leishmaniasis, as well as 76 sera from healthy controls. We found a sensitivity of 97% and a specificity of 89% in this assay. Some cross-reactions were seen, as observed in other immunoassays for the diagnosis of sporotrichosis. The ELISA appears to be especially useful for cutaneous forms of disease, since these are not promptly diagnosed with available immunoprecipitation or agglutination techniques. These results suggest that the ELISA using mycelial-phase S. schenckii exoantigens is a very sensitive diagnostic tool for the serodiagnosis of sporotrichosis and can be used in conjunction with conventional methods of diagnosis, particularly in cases where cross-reactions or false-positive results are experienced with the serodiagnosis.

* Corresponding author. Mailing address: Serviço de Micologia do Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Avenida Brasil, 4365, Manguinhos, 21045-900, Rio de Janeiro-RJ, Brazil. Phone: 55 21 3865-9557. Fax: 55 21 2590-9988. E-mail: zancope{at}ipec.fiocruz.br.

{triangledown} Published ahead of print on 10 January 2007.

Clinical and Vaccine Immunology, March 2007, p. 244-249, Vol. 14, No. 3
1071-412X/07/$08.00+0     doi:10.1128/CVI.00430-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Almeida-Paes, R., Pimenta, M. A., Monteiro, P. C. F., Nosanchuk, J. D., Zancope-Oliveira, R. M. (2007). Immunoglobulins G, M, and A against Sporothrix schenckii Exoantigens in Patients with Sporotrichosis before and during Treatment with Itraconazole. CVI 14: 1149-1157 [Abstract] [Full Text]  


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