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Clinical and Vaccine Immunology, March 2007, p. 211-219, Vol. 14, No. 3
1071-412X/07/$08.00+0     doi:10.1128/CVI.00306-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Effects of a Diphtheria-Tetanus-Acellular Pertussis Vaccine on Immune Responses in Murine Local Lymph Node and Lung Allergy Models{triangledown}

Rob J. Vandebriel,1* Eric R. Gremmer,1 Michiel van Hartskamp,1 Jan A. M. A. Dormans,1 and Frits R. Mooi2

Laboratory for Toxicology, Pathology, and Genetics,1 Laboratory for Vaccine-Preventable Diseases, National Institute of Public Health and the Environment, 3720 BA Bilthoven, The Netherlands2

Received 29 August 2006/ Returned for modification 11 October 2006/ Accepted 19 December 2006

We have previously shown that in mice, diphtheria-tetanus-acellular pertussis (DTaP) vaccination before Bordetella pertussis infection resulted in, besides effective clearance, immediate hypersensitivity (lung eosinophilia, increased total serum immunoglobulin E [IgE], and increased ex vivo Th2 cytokine production by cells from the bronchial lymph nodes). To better appreciate the extent of these findings, we measured DTaP vaccination effects in the local lymph node assay (LLNA) and an ovalbumin (OVA) lung allergy model. In the LLNA, mice were vaccinated or adjuvant treated before being sensitized with trimellitic anhydride (TMA; inducing a Th2-directed response) and dinitrochlorobenzene (DNCB; inducing a Th1-directed response). Compared to the adjuvant-treated controls, the vaccinated mice showed a decreased response to TMA and (to a much lesser extent) an increased response to DNCB. The decreased response to TMA coincided with increased transforming growth factor ß levels. With the exception of filamentous hemagglutinin, all vaccine constituents contributed to the decreased response to TMA. In the lung allergy model, sensitization induced OVA-specific IgE, lung pathology (peribronchiolitis, perivasculitis, and hypertrophy of the bronchiolar mucus cells) and increased the number of eosinophils, lymphocytes, and neutrophils in the bronchoalveolar lavage fluid. Vaccination failed to modulate these parameters. In conclusion, although DTaP vaccination may affect the LLNA response, we found no evidence of an effect on lung allergy.

* Corresponding author. Mailing address: Laboratory for Toxicology, Pathology and Genetics, National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands. Phone: 31-30-2742610. Fax: 31-30-2744437. E-mail: r.vandebriel{at}rivm.nl.

{triangledown} Published ahead of print on 3 January 2007.

Clinical and Vaccine Immunology, March 2007, p. 211-219, Vol. 14, No. 3
1071-412X/07/$08.00+0     doi:10.1128/CVI.00306-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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