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Clinical and Vaccine Immunology, December 2007, p. 1623-1628, Vol. 14, No. 12
1071-412X/07/$08.00+0     doi:10.1128/CVI.00158-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Antibody Detection and Kinetics of Antibody Production during Early Stages of Immunization with Hepatitis B Virus Vaccine{triangledown}

Odd Odinsen,1* Shirley Owusu-Ofori,2 Albert Dompreh,3 Francis Sarkodie,2 Ohene Opare-Sem,2 David Parker,4 and Jean-Pierre Allain5

PlasmAcute, Bergen, Norway,1 Department of Medicine, Transfusion Medicine Unit, Komfo Anokye Teaching Hospital, Kumasi, Ghana,2 Laboratory of Serology, Department of Microbiology, Komfo Anokye Teaching Hospital, Kumasi, Ghana,3 Nordiag ASA, Bergen, Norway,4 Department of Haematology, University of Cambridge, Cambridge, United Kingdom5

Received 12 April 2007/ Returned for modification 12 June 2007/ Accepted 28 September 2007

Antibodies to influenza virus and human immunodeficiency virus are detectable in B cells during the early stages of the immune response, prior to their occurrence in plasma. To investigate similar phenomena in a model of immunization against hepatitis B virus (HBV) infection, medical students in Ghana were screened for HBV markers, HBV surface (HBs) antigen (HBsAg), and HBV core antibodies (anti-HBc). Consenting volunteers, 24 of whom were seronegative (susceptible) and 2 of whom were positive for anti-HBc (prior infection), were vaccinated on day 0, day 40, and 6 months. Two sets of 10 blood samples, sequentially collected at intervals of 2 days following each immunization on days 0 and 40, were processed into B-cell lysates and plasma. Solid-phase HBsAg coated on microtiter plates for enzyme immunoassay or nitrocellulose membranes for dot blot assay was used to detect anti-HBs activity by an indirect antiglobulin assay. A commercially procured sandwich immunoassay was used, along with an enzyme-linked immunosorbent assay and a dot blot assay, for the detection of anti-HBs in B-cell lysates and plasma. Following the first injection of vaccine, a single sample of B-cell lysate collected between 5 and 21 days revealed anti-HBs in 18/21 subjects with no plasma antibodies detectable by sandwich immunoassay. After the booster dose was injected on day 40, a single sample of B-cell lysate collected between 44 and 49 days showed anti-HBs in 16/19 subjects, and this was accompanied by plasma antibodies in 8 subjects. In contrast, between 8 and 13 days, both subjects with prior HBV infection showed anti-HBs in B-cell lysates and plasma. Thus, primary immunization with the HBV vaccine appears to transiently elicit low-affinity anti-HBs in B-cell lysates into plasma.

* Corresponding author. Mailing address: PlasmAcute AS, High Technology Center, Thormøhlensgate 55, 5008 Bergen, Norway. Phone: 47 55 54 39 60. Fax: 47 55 54 38 98. E-mail: odd.odinsen{at}chello.no

{triangledown} Published ahead of print on 10 October 2007.

Clinical and Vaccine Immunology, December 2007, p. 1623-1628, Vol. 14, No. 12
1071-412X/07/$08.00+0     doi:10.1128/CVI.00158-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Odinsen, O., Parker, D., Radebe, F., Guness, M., Lewis, D. A (2009). Early Detection of Human Immunodeficiency Virus Type 1-Specific B-Lymphocyte-Derived Antibodies in a High-Risk Population. CVI 16: 1060-1065 [Abstract] [Full Text]  


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