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Clinical and Vaccine Immunology, October 2007, p. 1342-1348, Vol. 14, No. 10
1071-412X/07/$08.00+0     doi:10.1128/CVI.00168-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evaluation of a Single-Platform Technology for Lymphocyte Immunophenotyping{triangledown}

Jeanette Higgins,1* Valerie Hill,1 Karen Lau,2 Virginia Simpson,1 Jean Roayaei,3 Rick Klabansky,3 Randy A. Stevens,1 Julia A. Metcalf,4 and Michael Baseler1

AIDS Monitoring Laboratory, Clinical Services Program, SAIC-Frederick, Inc., NCI-Frederick, Frederick, Maryland 21702,1 Neutrophil Monitoring Laboratory, Clinical Services Program, SAIC-Frederick, Inc., NCI-Frederick, Frederick, Maryland 21702,2 Data Management Services, Inc., NCI-Frederick, Frederick, Maryland,3 National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 208924

Received 19 April 2007/ Returned for modification 25 May 2007/ Accepted 19 August 2007

An accurate and reproducible CD4 count is a fundamental clinical tool for monitoring and treating human immunodeficiency virus infection and its complications. Two methods exist for calculating absolute CD4 counts: dual-platform technology (DPT) and single-platform technology (SPT). Numerous studies have documented the unacceptably wide range of variation in absolute CD4 counts between laboratories. SPT was introduced in 1996 to reduce the interlaboratory variation in absolute CD4 counts. The aim of this study was to compare DPT with the BD Biosciences Trucount method (an SPT method). Both the percentages of CD4 (r = 0.986; P = 0.0541) and the absolute CD4 counts (r = 0.960; P = 0.0001) had very good correlation between the two methods. However, poor correlation was observed for the CD8+ RO– (r = 0.314; P = 0.0002), CD8+ DR+ (r = 0.666; P = 0.0138), CD3+ CD38+ (r = 0.8000; P = 0.0004), CD3+ CD25+ (r = 0.464; P = 0.0082), and CD4+ CD38+ (r = 0.357; P = 0.0127) measurements.

* Corresponding author. Mailing address: AIDS Monitoring Laboratory, Clinical Services program, SAIC-Frederick, Inc., NCI-Frederick, P.O. Box B, Bldg. 469-3 Room 9, Frederick, MD 21702. Phone: (301) 846-6267. Fax: (301) 846-5224. E-mail: jhiggins{at}mail.nih.gov

{triangledown} Published ahead of print on 29 August 2007.

Clinical and Vaccine Immunology, October 2007, p. 1342-1348, Vol. 14, No. 10
1071-412X/07/$08.00+0     doi:10.1128/CVI.00168-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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