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Clinical and Vaccine Immunology, October 2007, p. 1334-1341, Vol. 14, No. 10
1071-412X/07/$08.00+0     doi:10.1128/CVI.00181-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Differential B-Cell Responses Are Induced by Mycobacterium tuberculosis PE Antigens Rv1169c, Rv0978c, and Rv1818c{triangledown}

Yeddula Narayana,1 Beenu Joshi,2 V. M. Katoch,2 Kanhu Charan Mishra,1 and Kithiganahalli N. Balaji1*

Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012,1 National Jalma Institute of Leprosy and Other Mycobacterial Diseases, Tajganj, Agra 282-001, India2

Received 26 April 2007/ Returned for modification 29 May 2007/ Accepted 27 July 2007

The multigene PE and PPE family represents about 10% of the genome of Mycobacterium tuberculosis. Here, we report that three members of the PE family, namely, Rv1169c, Rv0978c, and Rv1818c, elicit a strong, but differential, B-cell humoral response among different clinical categories of tuberculosis patients. The study population (n = 211) was comprised of different clinical groups of both adult and child patients: group 1 (n = 94) patients with pulmonary infection, group 2 (n = 30) patients with relapsed infection, group 3 (n = 31) patients with extrapulmonary infections, and clinically healthy donors (n = 56). Among the PE proteins studied, group 1 adult patient sera reacted to Rv1818c and Rv0978c, while Rv1169c elicited immunoreactivity in group 3 children. However, all three PE antigens studied as well as the 19-kDa antigen did not demonstrate humoral reactivity with sera from group 2 patients with relapsed infection. The current study shows that while responsiveness to all three PE antigens is a good marker for M. tuberculosis infection, a strong response to Rv0978c or to Rv1818c by group 1 adult patients with pulmonary infection or largely restricted reactivity to Rv1169c antigen in child patients with extrapulmonary infections offers the possibility of differential utility in the serodiagnosis of tuberculosis.

* Corresponding author. Mailing address: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India. Phone: 91-80-22933223. Fax: 91-80-23602697. E-mail: balaji{at}mcbl.iisc.ernet.in

{triangledown} Published ahead of print on 8 August 2007.

Clinical and Vaccine Immunology, October 2007, p. 1334-1341, Vol. 14, No. 10
1071-412X/07/$08.00+0     doi:10.1128/CVI.00181-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Koh, K. W., Soh, S. E, Seah, G. T. (2009). Strong Antibody Responses to Mycobacterium tuberculosis PE-PGRS62 Protein Are Associated with Latent and Active Tuberculosis. Infect. Immun. 77: 3337-3343 [Abstract] [Full Text]  


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