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Clinical and Vaccine Immunology, January 2007, p. 110-113, Vol. 14, No. 1
1071-412X/07/$08.00+0     doi:10.1128/CVI.00289-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Accuracy of Enzyme-Linked Immunosorbent Assay Using Crude and Purified Antigens for Serodiagnosis of Melioidosis{triangledown}

Narisara Chantratita,1* Vanaporn Wuthiekanun,1 Aunchalee Thanwisai,1 Direk Limmathurotsakul,1 Allen C. Cheng,2 Wirongrong Chierakul,1 Nicholas P. J. Day,1,3 and Sharon J. Peacock1,3

Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand,1 Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia,2 Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom3

Received 8 August 2006/ Returned for modification 13 October 2006/ Accepted 23 October 2006

Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).

* Corresponding author. Mailing address: Wellcome Unit, Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok, 10400, Thailand. Phone: 66 2 354 1395. Fax: 66 2 354 9169. E-mail: narisara{at}tropmedres.ac.

{triangledown} Published ahead of print on 8 November 2006.

Clinical and Vaccine Immunology, January 2007, p. 110-113, Vol. 14, No. 1
1071-412X/07/$08.00+0     doi:10.1128/CVI.00289-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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Copyright © 2007 by the American Society for Microbiology. All rights reserved.