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Clinical and Vaccine Immunology, June 2006, p. 704-707, Vol. 13, No. 6
1071-412X/06/$08.00+0     doi:10.1128/CVI.00417-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Monocyte CD163 and CD36 Expression in Human Whole Blood and Isolated Mononuclear Cell Samples: Influence of Different Anticoagulants

Marcin Moniuszko,1* Krzysztof Kowal,1 Malgorzata Rusak,2 Miroslawa Pietruczuk,2 Milena Dabrowska,2 and Anna Bodzenta-Lukaszyk1

Department of Allergology and Internal Medicine, Medical University of Bialystok, 15-276 Bialystok, Poland,1 Department of Hematological Diagnostics, Medical University of Bialystok, 15-274 Bialystok, Poland2

Received 10 December 2005/ Returned for modification 2 February 2006/ Accepted 11 April 2006

We investigated whether the choice of anticoagulant or the application of density gradient mononuclear cell isolation may account for conflicting published data regarding the levels of the scavenger receptors' expression in healthy individuals. We demonstrate that the detection of CD163, but not CD36, differs dramatically among the methods.

* Corresponding author. Mailing address: Department of Allergology and Internal Medicine, Medical University of Bialystok, Sklodowskiej-Curie Street 24A, 15-276 Bialystok, Poland. Phone: 48 509 138 579. Fax: 48 85 74 68 601. E-mail: moniuszm{at}amb.edu.pl.

Clinical and Vaccine Immunology, June 2006, p. 704-707, Vol. 13, No. 6
1071-412X/06/$08.00+0     doi:10.1128/CVI.00417-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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