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Clinical and Vaccine Immunology, June 2006, p. 698-701, Vol. 13, No. 6
1071-412X/06/$08.00+0     doi:10.1128/CVI.00071-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Expression of Bovine Viral Diarrhea Virus Glycoprotein E2 as a Soluble Secreted Form in a Mammalian Cell Line

Gaetano Donofrio,1* Ezio Bottarelli,2 Cavirani Sandro,1 and Cesidio Filippo Flammini1

Dipartimento di Salute Animale, Sezione di Malattie Infettive degli Animali,1 Sezione di Microbiologia, Facoltà di Medicina Veterinaria, via del Taglio 8, 43100 Parma, Italy2

Received 23 February 2006/ Returned for modification 27 March 2006/ Accepted 29 March 2006

Bovine viral diarrhea virus (BVDV) membrane-anchored type I glycoprotein E2 is an ~53-kDa immunodominant glycoprotein inducing the production of neutralizing antibodies in the animal host after natural infection or following immunization with live or killed vaccines. The E2 coding region lacking the transmembrane domain was constructed in a soluble secreted form (secE2) and expressed in the medium of a transiently transfected human cell line. The crude conditioned medium containing secE2 can be potentially employed to develop an enzyme-linked immunosorbent assay antigen for the diagnosis of BVDV infection or for vaccine purposes.

* Corresponding author. Mailing address: Dipartimento di Salute Animale, Sezione di Malattie Infettive degli Animali, Facoltà di Medicina Veterinaria, Via del Taglio 8, 43100 Parma, Italy. Phone: 00390521902677. Fax: 00390521902672. E-mail: gaetano.donofrio{at}unipr.it.

Clinical and Vaccine Immunology, June 2006, p. 698-701, Vol. 13, No. 6
1071-412X/06/$08.00+0     doi:10.1128/CVI.00071-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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