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Clinical and Vaccine Immunology, June 2006, p. 640-647, Vol. 13, No. 6
1071-412X/06/$08.00+0     doi:10.1128/CVI.00024-06

Correlation of Cytokine Gene Expression with Pathology in White-Tailed Deer (Odocoileus virginianus) Infected with Mycobacterium bovis

Tyler C. Thacker,* Mitchell V. Palmer, and W. Ray Waters

United States Department of Agriculture, Agricultural Research Service, Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Ames, Iowa 50010

Received 19 January 2006/ Returned for modification 21 February 2006/ Accepted 11 April 2006

Mycobacterium bovis-infected white-tailed deer (WTD) in northeast Michigan are a reservoir of mycobacteria that pose a threat to both domestic animals and humans. Relatively little work has been done to characterize the immune response of WTD to M. bovis infection; however, an understanding of the immune response to infection and pathogenesis may be critical to the development of an effective vaccine. Immunological responses to infection were characterized by monitoring cytokine gene expression in M. bovis-infected and uninfected deer. Peripheral blood leukocytes (PBL) from infected WTD expressed more gamma interferon (IFN-{gamma}), interleukin-12p40 (IL-12p40), granulocyte-monocyte colony-stimulating factor, and IL-4 mRNA than did PBL from uninfected deer; however, differences were not detected in expression of IL-10 and transforming growth factor-ß mRNA. Infected animals could be divided into two groups based on pathology. Lesions were confined primarily to the lymph nodes of the head in animals with less severe pathology. Animals with more severe pathology had lesions in the lung and associated lymph nodes as well as the lymph nodes of the head. More robust IFN-{gamma} mRNA expression correlated with pathology early in infection. These findings indicate that IFN-{gamma} expression likely plays a role in both protection and pathogenesis.

* Corresponding author. Mailing address: National Animal Disease Center, Bacterial Diseases of Livestock Research Unit, 2300 Dayton Ave., Ames, IA 50010. Phone: (515) 663-7294. Fax: (515) 663-7458. E-mail: tthacker{at}nadc.ars.usda.gov.

Clinical and Vaccine Immunology, June 2006, p. 640-647, Vol. 13, No. 6
1071-412X/06/$08.00+0     doi:10.1128/CVI.00024-06




This article has been cited by other articles:

  • Thacker, T. C., Palmer, M. V., Waters, W. R. (2009). T-Cell mRNA Expression in Response to Mycobacterium bovis BCG Vaccination and Mycobacterium bovis Infection of White-Tailed Deer. CVI 16: 1139-1145 [Abstract] [Full Text]  
  • Robinson, M., O'Brien, R., Mackintosh, C., Griffin, F. (2008). Differential Immune Responses of Red Deer (Cervus elaphus) following Experimental Challenge with Mycobacterium avium subsp. paratuberculosis. CVI 15: 963-969 [Abstract] [Full Text]  


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