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Clinical and Vaccine Immunology, April 2006, p. 520-524, Vol. 13, No. 4
1071-412X/06/$08.00+0     doi:10.1128/CVI.13.4.520-524.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

One-Step Immunochromatography Assay Kit for Detecting Antibodies to Canine Parvovirus

Jin-Sik Oh,1 Gun-Woo Ha,1 Young-Shik Cho,1 Min-Jae Kim,2 Dong-Jun An,3 Kyu-Kye Hwang,4 Yoon-Kyu Lim,4 Bong-Kyun Park,5 BoKyu Kang,6 and Dae-Sub Song6*

Animal Genetics, Inc., Suwon, Kyunggi-do 440-290, Korea,1 KimMinJae Animal Hospital, Seoul 138-858, Korea,2 National Veterinary Research and Quarantine Service, Anyang, Kyunggi-do 430-824, Korea,3 Department of Veterinary Medicine, Cheju National University, Cheju 690-756, Korea,4 Department of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea,5 Research Unit, Green Cross Veterinary Products, Yongin 227-5, Korea6

Received 5 October 2005/ Returned for modification 14 November 2005/ Accepted 12 January 2006

This study was performed to determine the feasibility of using whole serum to detect antibodies to canine parvovirus (CPV) under nonlaboratory conditions and to evaluate the performance characteristics of an immunochromatography assay kit. Precise detection of levels of antibody against CPV in puppies can be used to determine a vaccination schedule, because maternal antibodies frequently result in the failure of protective vaccination, and can also be used to determine the antibody levels of infected puppies. Several methods for the titration of CPV antibodies have been reported, including the hemagglutination inhibition (HI) assay, which is considered the "gold standard." These methods, however, require intricate and time-consuming procedures. In this study, a total of 386 serum specimens were tested. Compared to the HI assay, the rapid assay had a 97.1% sensitivity and a 76.6% specificity (with a cutoff HI titer of 1:80). This single-step assay could be performed rapidly and easily without special equipment. The kit provides a reliable method for detection of anti-CPV antibody where laboratory support and personnel are limited.

* Corresponding author. Mailing address: Research Unit, Green Cross Veterinary Products, Yongin 227-5, Korea. Phone: 82-31-283-3423. Fax: 82-31-281-8814. E-mail: sds{at}snu.ac.kr.

Clinical and Vaccine Immunology, April 2006, p. 520-524, Vol. 13, No. 4
1071-412X/06/$08.00+0     doi:10.1128/CVI.13.4.520-524.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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