This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mendelson, M.
Right arrow Articles by Kaplan, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mendelson, M.
Right arrow Articles by Kaplan, G.

Next Article 

Clinical and Vaccine Immunology, December 2006, p. 1299-1306, Vol. 13, No. 12
1071-412X/06/$08.00+0     doi:10.1128/CVI.00132-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Quantitative and Functional Differences between Peripheral Blood Myeloid Dendritic Cells from Patients with Pleural and Parenchymal Lung Tuberculosis{triangledown}

Marc Mendelson,1,2* Willem A. Hanekom,2 Siyabulela Ntutela,2 Monica Vogt,2 Lafras Steyn,2 Gary Maartens,2 and Gilla Kaplan1

Laboratory of Mycobacterial Immunity and Pathogenesis, Public Health Research Institute, International Center for Public Health, Newark, New Jersey 07103-3535,1 Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town 7925, South Africa2

Received 5 April 2006/ Returned for modification 8 June 2006/ Accepted 20 September 2006

Dendritic cells (DCs) play a pivotal role in generating protective host immunity to Mycobacterium tuberculosis. Few studies have addressed DC function in the context of active tuberculosis (TB), largely due to technical constraints in obtaining adequate numbers of DC from sick patients. We quantitated peripheral blood myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) in the whole blood of patients with active TB and show that blood from patients with pleural TB was characterized by high circulating levels of mDCs. We also developed and optimized a novel whole-blood assay to study mDC production of the Th1-promoting cytokine interleukin 12 (IL-12) and upregulation of the maturation marker CCR7 after incubation with mycobacteria. We found that pleural TB was associated with increased IL-12 production and CCR7 expression compared to lung parenchymal disease. Our findings suggest important differences in innate immunity between patients with different forms of active TB, and this may contribute to the differences in natural history observed between the two groups.

* Corresponding author. Mailing address: Institute of Infectious Diseases and Molecular Medicine, Wernher Beit South Building, Room S2.16, Anzio Rd., Observatory 7925, Cape Town, South Africa. Phone: 27 21 406 6793. Fax: 27 21 448 8150. E-mail: mmendels{at}curie.uct.ac.za.

{triangledown} Published ahead of print on 27 September 2006.

Clinical and Vaccine Immunology, December 2006, p. 1299-1306, Vol. 13, No. 12
1071-412X/06/$08.00+0     doi:10.1128/CVI.00132-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»